Wolbachia感染导致果蝇dHira基因表达下调  

Infection of Wolbachia caused down-regulation of dHira in Drosophila melanogaster

作　　者：陈丽娟[1] 郑雅[1] 王玉凤[1] 曾庆韬[2] 刘艳玲[2] 

机构地区：[1]遗传调控与整合生物学湖北省重点实验室,华中师范大学生命科学学院,武汉430079 [2]湖北大学生命科学学院,武汉430062

出　　处：《动物学报》2008年第4期748-752,共5页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金(30671609);教育部聘请外籍教师重点项目[教外司专(2007) 364号]资助项目~~

摘　　要：Intracellular Wolbachia bacteria are obligate, maternally-inherited, endosymbionts found frequently in arthropods and other invertebrates. The wide occurrence of Wolbachia may be partially due to their ability to alter host reproduction via mechanisms including cytoplasmic incompatibility (CI), parthenogenesis, feminization and male killing. The molecular mechanisms of Wolbachia/host interaction remain to be elucidated. In this study, Drosophila infected with Wolbachia collected from Wuhan (Hubei province), Liuku (Yunnan Province) and Tianjing, China were treated with tetracycline to delete Wolbachia and the transcriptional expression of dHira was analysed by RT-PCR and quantitative RT-PCR to assay the effect of Wolbachia infection on hosts. The dHira transcript level was significantly increased (P<0.01)after depletion of Wolbachia in D.melanogaster. This indicates that the infection of Wolbachia may down-regulate the transcription of dHira. Considering that dHira is involved in the decondensation of the sperm nucleus after fertilization and the formation of a normal male pronucleus, we suggest that Wolbachia may affect the host reproduction, at least partially, via the dHira pathway .Intracellular Wolbachia bacteria are obligate, maternally-inherited, endosymbionts found frequently in arthropods and other invertebrates. The wide occurrence of Wolbachia may be partially due to their ability to alter host reproduction via mechanisms including cytoplasmic incompatibility （CI）, parthenogenesis, feminization and male killing. The molecular mechanisms of Wolbachia/host interaction remain to be elucidated. In this study, Drosophila infected with Wolbachia collected from Wuhan （Hubei province）, Liuku （Yunnan Province） and Tianjing, China were treated with tetracycline to delete Wolbachia and the transcriptional expression of dHira was analysed by RT-PCR and quantitative RT-PCR to assay the effect of Wolbachia infection on hosts. The dHira transcript level was significantly increased （ P 〈 0.01 ） after depletion of Wolbachia in D. melanogaster. This indicates that the infection of Wolbachia may down-regulate the transcription of dHira. Considering that dHira is involved in the decondensation of the sperm nucleus after fertilization and the formation of a normal male pronucleus, we suggest that Wolbachia may affect the host reproduction, at least partially, via the dHira pathway

关 键 词：果蝇 Wolbachia感染 dHira 

分 类 号：S476[农业科学—农业昆虫与害虫防治] Q96[农业科学—植物保护]