初发系统性红斑狼疮患者调节性T细胞的变化  

Regulatory T cells in patients with early new-onset systemic lupus erythematosus

作　　者：张飚[1] 张烜[1] 唐福林[1] 朱立平[2] 刘音[2] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院风湿免疫科 [2]中国医学科学院北京协和医学院基础医学研究所免疫学系,北京100005

出　　处：《基础医学与临床》2008年第9期920-924,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(30400410);教育部新世纪优秀人才支持计划(NCET-04-0191);北京市自然科学基金(7052052)

摘　　要：目的探讨初发系统性红斑狼疮(SLE)患者CD4+T细胞中FOXP3和CD25的表达及其临床意义。方法应用流式细胞仪检测CD4+FOXP3+、CD4+CD25+、CD4+CD25highT细胞表达,同时检测CD4+FOXP3+T细胞中CD25及CD4+CD25highT细胞中FOXP3的表达。结果活动组SLE患者CD4+CD25+T细胞显著低于对照组和低活动组(P<0.05)。活动组患者CD4+CD25highT细胞明显低于低活动组患者(P<0.01)。初发SLE患者CD4+FOXP3+T细胞明显高于对照组(P<0.01)。活动组SLE患者CD4+FOXP3+T细胞中CD25表达显著低于对照组和低活动组(P<0.05)。同时活动组SLE患者CD4+CD25highT细胞中FOXP3表达的平均荧光强度较对照组显著降低(P<0.05)。活动组患者治疗后CD4+CD25highT细胞显著增高(P<0.05)。结论初发活动期SLE患者CD4+T细胞中CD25high和FOXP3表达不一致可能在SLE患者免疫失衡中发挥作用。Objective To investigate the expression of FOXP3 and CD25 in CD4^＋ T cells of peripheral blood from patients with early new-onset systemic lupus erythematosus（SLE） before and after treatment. Methods Forty-four early new-onset SLE patients including twenty-four with active disease （SLEDAI ≥ 10）, twenty with low active disease （SLEDAI 〈 5 ） were enrolled in this study. Twenty-one healthy volunteers were also collected as controls. Peripheral blood samples before and after treatment were sampled. Cell populations of CD4^＋ CD25 ^＋ T cells, CD4^＋ CD25^highT cells and CD4 ^＋ FOXP3 ^＋ T ceils was quantified and the expression of CD25 in CD4 ^＋ FOXP3 ^＋ T cells and FOXP3 in CD4^＋ CD25^highT cells were also analyzed. Results Peripheral blood CD4 ^＋ CD25 ^＋T cells and CD4 ^＋ CD25^highT cells in active new-onset lupus patients （3.95% - 13.04%, 0. 04% - 1.34% ） were significantly lower than inlowactivelupuspatients （ 7.27 % - 24.48 % , 0. 14 % - 3.07 % ） （ P 〈 0.05, P 〈 0. 01 ） ; Though there was no significantly difference of CD4^＋ FOXP3^ ＋T ceils in active （7.46% - 17. 38% ） and in low active （5.30% - 23.00% ） new-onset lupus patients, both were significantly higher than in normal controls（2. 51% - 12. 94% ） （P 〈 0. 01 ,P 〈0. 01 ） ; CD25 expression in CD4^ ＋ FOXP3^＋ T cells in active lupus patients （25. 24% - 62. 47% ） was significantly lower than in low active untreated lupus patients （30. 35% - 75.25% ） and normal controls （54. 83% - 86. 38% ） （P 〈0. 05, P 〈0. 05）. Meanwhile, Mean fluorescent intensity （MFI） of FOXP3 in CD4 ^＋ CD25^highT cells from patients with new-onset SLE was significantly lower than from normal control （100. 52 - 160. 92 vs 122. 58 - 198. 10, P =0. 012）. CD4^＋ CD25^highT ceils increased significantly in active lupus patients after corticosteroid treatment（P 〈0. 05）. Conclusion The disproportional expression between CD25^high and FOXP3 ^＋ in CD4^ ＋T cells may play an importa

关 键 词：系统性红斑狼疮 CD4^+CD25^+调节性T细胞 FOXP3 

分 类 号：R593.241[医药卫生—内科学]