脂联素基因+45位T/G多态性与2型糖尿病遗传易感性的相关性  

SNP 45(T/G) of adiponectin gene polymorphism and genetic susceptibility to type 2 diabetes mellitus

作　　者：魏颖丽 何兰杰[2] 霍正浩[3] 赵巍[3] 

机构地区：[1]浙江武警总队医院杭州医院,浙江杭州310000 [2]宁夏医学院附属医院内分泌科 [3]宁夏医学院医学遗传学与细胞生物学教研室,宁夏银川750004

出　　处：《基础医学与临床》2008年第9期940-943,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30260045);宁夏自然科学基金(A4005)

摘　　要：目的研究脂联素基因单核苷酸多态性(SNP)45(T/G)位点与宁夏汉族人群2型糖尿病之间的关系。方法100例2型糖尿病患者和101例正常对照者,采用聚合酶链式反应——限制性内切酶长度多态性(PCR-RFLP)技术,对脂联素基因SNP45多态性位点进行基因分型,同时测定代谢参数。结果2型糖尿病组SNP45位点GG基因型频率和G等位基因频率均高于正常对照组(P<0.05)。结论脂联素基因的SNP45多态性位点与宁夏汉族人群中2型糖尿病相关,GG基因型者具有2型糖尿病高易感性。Objective To investigate the relation of single nucleotide polymorphisms （SNP）45T/G the adiponectin gene with type 2 diabetes of Han nationality in Ningxia. Methods PCR-RFLP method was used to identify the distribution of alleles and genotypes frequencies of SNP45T/G polymorphisms in adiponectin gene in 100 type 2 diabetic patients and 101 normal subjects. Results The distribution of genotypes and alleles frequencies of the SNP45 polymorphisms were significantly different （GG genotype： 13% vs 3%, P 〈 0.05; G allele; 76% vs 24%, P 〈 0. 05）. Conclusion SNP45 in adiponectin gene was related to type 2 diabetes of Han nationality in Ningxia. Individuals with SNP45 GG genotype of adiponectin gene have high susceptibility to type 2 diabetes.

关 键 词：2型糖尿病 脂联素 基因 单核苷酸多态性 遗传易感性 

分 类 号：R587.1[医药卫生—内分泌]