麦长管蚜ELISA检测体系的建立  

Establishment of a suitable enzyme-linked immunosorbent assay(ELISA) for Macrosiphum avenae

作　　者：高书晶[1] 庞保平[1] 陈正贤[2] 

机构地区：[1]内蒙古农业大学农学院 [2]浙江大学生物技术研究所,杭州310029

出　　处：《昆虫知识》2008年第5期730-735,共6页Entomological Knowledge

基　　金：国家自然科学基金项目(30160051);中央级公益性科研院基本科研业务费专项资金(中国农业科学院草原研究所)资助项目

摘　　要：应用麦长管蚜Macrosiphum avenae(Fabricius)单克隆抗体比较直接、间接和夹心ELISA方法。结果表明,间接ELISA的灵敏度高于直接ELISA,灵敏度分别为0.042μg/mL和0.083μg/mL,而双抗夹心ELISA灵敏度很差,无法使用。建立用于检测捕食性瓢虫肠道内麦长管蚜抗原的间接ELISA检测体系,为进一步应用单克隆抗体评价捕食性瓢虫对麦长管蚜的控制作用奠定可靠的基础。Direct,indirect and double-antibody-sandwich enzyme-linked immunosorbent assay（ELISA） were examined with the monoclonal antibodies to detect the antigen of Macrosiphum avenae（Fabricius） in the gut of predatory ladybirds.The results showed indirect ELISA was more sensitively than direct ELISA,in which the detection sensitivities were 0.042 μg/mL and 0.083 μg/mL,respectively wheres double antibody sandwich ELISA was not sensitively to be used.An indirect ELISA method was set up in order to detect the antigen of M.avenae in the gut of predatory ladybirds,which established a reliable foundation for evaluating the predation of predatory ladybirds on M.avenae with the monoclonal antibodies.

关 键 词：麦长管蚜 单克隆抗体 酶联免疫吸附试验 灵敏度 

分 类 号：S433[农业科学—农业昆虫与害虫防治]