铁调素对成骨细胞内铁离子转运的影响  

A study on the influence of hepcidin out of osteoblasts on iron transportation

作　　者：赵东阳[1] 徐又佳[1] 张鹏[1] 郝彦明[1] 马勇[1] 王冰[1] 

机构地区：[1]苏州大学附属第二医院骨科,215004

出　　处：《中国骨质疏松杂志》2008年第9期611-614,共4页Chinese Journal of Osteoporosis

基　　金：江苏省教育厅自然基金课题(05KJB320125);国际合作计划(SWH0617);苏州大学医学发展基金(EE126621);江苏省医学重点人才项目(RC2007100)

摘　　要：目的研究不同时间和不同浓度铁调素（Hepcidin）对人成骨细胞（hFOB 1.19）内铁离子影响。方法使用Hepcidin干预hFOB 1.19细胞后,采用激光共聚焦扫描显微镜（CLSM）观察成骨细胞内的铁离子荧光强度。结果CLSM扫描细胞内不同时间组铁离子荧光强度显示：①瞬时组内,实验组与对照组荧光强度均出现缓慢下降,二者之间变化趋势无明显的差异。②长时间组内,在0-100 nmol/L范围内,细胞内铁离子荧光强度随着Hepcidin浓度增加而逐渐增强,当Hepcidin的浓度超过100 nmol/L时,细胞内的铁离子荧光强度不再随着Hepcidin浓度的增加而继续增强。结论①Hepcidin对hFOB1.19内铁离子瞬时转运的作用没有明显的影响。②Hepcidn干预hFOB 1.19细胞20 h后有升高细胞内的铁离子含量的作用,并且在0-100 nmol/L范围内具有剂量依赖性。Objective To study the influence of Hepcidin of different concentration out of hFOB 1.19 cells on iron transportation in the different time. Methods Use Hepcidin to impact the hFOB 1.19 cells, and the fluorescence intensity of iron was observed by confocal laser scanning microscope （CLSM）. Results ①The transient group： both of the fluorescence intensity of iron decreased slowly in the control group and the experiment group, there was no significant difference between them. ②The long-time group： At the range of 0 - 100 nmol/L, the fluorescence intensity of iron ion in hFOB 1.19 was increased with the increase of Hepeidin concentration out of cells. When the Hepcidin concentration exceeded 100 nmol/L, the fluorescence intensity of iron did not increase anymore. Conclusion ① The instantaneous influence of Hepcidin on iron transportation is not significant. ②The iron concentration was increased significantly by adding Hepcidin out of hFOB 1.19 cells 20 hours later, and there was a dose dependent relationship between them when the Hepcidin concentration is in the range of 0 - 100 nmol/L.

关 键 词：铁调素 铁离子 成骨细胞 骨质疏松 

分 类 号：R336[医药卫生—人体生理学]