日本脑炎病毒受体功能缺陷细胞与亲代细胞膜分子差异的鉴定  

Identification of Differentially Expressed Membrane Proteins between Mutant 3A10-3F Cells Lacking the Function of Japanese Encephalitis Virus (JEV) Receptor and Their Parental Cells BHK-21

作　　者：任君萍[1] 马文煜[1] 丁天兵[1] 

机构地区：[1]第四军医大学基础部微生物学教研室,西安710032

出　　处：《科学技术与工程》2008年第18期5155-5160,共6页Science Technology and Engineering

基　　金：国家自然科学基金(30600526;30470091)资助

摘　　要：鉴定日本脑炎病毒(JEV)受体功能缺陷细胞3A10-3F与亲代细胞BHK-21膜分子表达的差异,以发现JEV受体候选分子。3A10-3F与BHK-21细胞膜蛋白用氯仿去脂法分别提取,通过二维电泳(2-DE)分离。经ImageMaster 2D软件分析比较后,选取差异点做液质联用质谱分析(LC-MS/MS)。发现有23个蛋白点在两组2-DE凝胶中有显著差异表达,LC-MS/MS鉴定了14个蛋白质,其中有4个膜蛋白,即钙结合蛋白annexin 1,annexin 2;电压依赖的离子通道(VDACs)蛋白VDAC1,VDAC2。上述结果为深入鉴定JEV受体分子特性与功能,阐明JEV致病机理提供了线索。To identify differentially expressed membrane proteins between 3A10-3F cells lacking the function of Japanese encephalitis virus （JEV） receptor and parental BHK-21 cells, hoping to provide clues to unveil the putative JEV receptors proteins, membrane-associated proteins from 3A10-3F and BHK-21 cells were obtained by the method of chloroform delipidation, and separated by two-dimensional electrophoresis （2-DE） respectively. The differential spots were analyzed with ImageMaster 2D software and identified by LC-MS/MS. There were twenty-three spots differentially expressed in 3A10-3F and BHK-21 cells gels. Fourteen proteins were identified by LC-MS/MS, and four of them were membrane proteins, which were annexin A1 and annexin A2, voltage-dependent anion channel （VDAC） 1 and 2. These data may provide important clues for identify the characteristics and function of JEV receptors and elucidate JEV pathogenesis.

关 键 词：日本脑炎病毒(JEV) 受体 二维电泳(2-DE) 质谱(MS) 

分 类 号：R373.31[医药卫生—病原生物学]