棘刺锚参抗肿瘤因子抗肿瘤作用的实验研究  

Experimental study of effects of Protankyra Bidentata anti-tumor factor on tumor growth

作　　者：沈先荣[1] 栾洁[1] 蒋定文[1] 陈伟[1] 刘李娜[1] 陆敏[1] 贾福星[1] 

机构地区：[1]海军医学研究所,上海200433

出　　处：《中华航海医学与高气压医学杂志》2008年第4期200-203,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：国家自然科学基金项目（30471972）

摘　　要：目的研究棘刺锚参抗肿瘤因子（Protankyra Bidentata anti—tumorfactor，PBATF）在动物水平的抗肿瘤作用。方法采用小鼠H22肝癌模型、大鼠Walker-256癌肉瘤模型、人体LAX肺腺癌模型、人体QGY肝癌模型，分别测定PBATF对不同肿瘤生长的抑制效应。各种模型分别设PBATF高（5mg／kg）、中（1mg／kg）、低（0．5ms／kg）剂量组、阳性对照组（CTX）和阴性对照组。试验组及阳性对照组昆明种小鼠或Wistar大鼠每组均10只，裸小鼠每组均6只，阴性对照组每次各为2组。腹腔注射给药，每天1次，连续7～10d。采用常规方法测定PBATF腹腔注射的LD50。结果PBATF对大鼠Walker．256癌肉瘤的抑瘤率，高剂量组62．63％、中剂量组52．31％、低剂量37．49％。PBATF对人体LAX肺腺癌的抑瘤率，高剂量组56．02％、中剂量组50．60％、低剂量组40．36％。PBATF对人体QGY肝癌的抑瘤率，高剂量组52．07％、中剂量组45．56％、低剂量组33．14％。PBATF对小鼠H22肝癌的抑瘤率，高剂量组59．25％、中剂量组57．74％、低剂量组45．28％。PBATF腹腔注射给药，小鼠的LD50为1．973g／kg。结论获得了高纯度的PBATF，能够显著抑制体内外肿瘤生长。Objective To study the inhibitory effects of Protankyra Bidentata anti-tumor factor （PBATF） on the growth of tumors implanted in mice or rats. Methods Methods of H22 liver cancer implanted in Kunming mice, Walker-256 sarcoma implanted in Wistar rats, human LAX lung cancer and human QGY liver cancer implanted in nest mice were used to detect the inhibitory effects of PBATF on the tumor growth, respectively. Animals in each model were divided into 5 groups：high PBATF group （5 mg/kg） , middle PBATF group（ 1 mg/kg）, low PBATF group（0.5 mg/kg）, positive control group （CTX） and negative control group. The preparations were administrated once a day, for 7 - 10 days. The LD50 peritoneal injection of PBATF was detected according to ordinary method. Results The inhibitory tumor rates of PBATF on Walker-256 sarcoma implanted in Wistar rats, human LAX lung cancer implanted in nest mice, human QGY liver cancer implanted in nest mice and QGY liver cancer implanted in nest mice were 62.63% ,56.02% ,52. 07% and 59.25% in high dose group, 52. 31% ,50. 60% ,45. 56% and 57.74% in middle dose group, 37.49% , 40.36% , 33. 14% and 45.28% in low dose group, respectively. The LD50 peritoneal injection of PBATF was 1. 973 g/kg. Conclusions PBATF has significant inhibitory effect on tumor growth in vivo.

关 键 词：棘刺锚参 肝癌 肺腺癌 癌肉瘤 肿瘤治疗 

分 类 号：R686[医药卫生—骨科学]