沙土鼠短时氧惊厥海马各区神经元c—fos蛋白表达的变化  

Changes in the expression of c-fos protein in the hippocampus following transient hyperbaric oxygeninduced convulsion in gerbils

作　　者：周建光[1] 刘长云[1] 方以群[2] 周颖奇[1] 徐尔理[1] 周春[1] 刘景昌[2] 方霞[1] 

机构地区：[1]解放军第四一一医院,上海200081 [2]海军医学研究所

出　　处：《中华航海医学与高气压医学杂志》2008年第4期211-213,共3页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的观察短时氧惊厥沙土鼠脑海马各区神经元c—fos蛋白的表达，探讨氧惊厥时神经元激活部位。方法18只健康雄性沙土鼠经观察和检疫1周后进行实验。按体质量采用随机数字法分为3组（正常对照组、短时氧惊厥组、压力对照组），每组3只。沙土鼠暴露在0．5MPa压力的高压氧下，引起短时氧惊厥（首次惊厥5min后，减压出舱）后应用LSAB染色法观察海马各区神经元c—fos蛋白的表达情况。结果沙土鼠在发生短时氧惊厥后，用免疫组化染色的脑切片上发现海马各区神经元c—fns蛋白阳性表达，依次为CA1、CA2、CA4、CA3区。结论海马是氧惊厥时神经元激活的部位之一，海马CA1区作用最强。Objective To observe changes in the expression of e-fos protein in different regains of gerbil hippocampus following transient hyperbaric oxygen-induced convulsion （HBOC） and to explore neuronal localization of the HBOC. Methods Eighteen healthy mail gerbils were used and experiments were carried out after 1 week of observation and quarantine. Gerbils were divided by random number table into normal control, transient convulsion and hyperbaric oxygen （HBO） control groups with 3 gerbils in each group. Transient oxygen convulsion was induced by 0.5 MPa HBO（ gerbils were taken out 5 min later following first convulsion） , then morphological changes of neurons in hippoeampus were obsered by LSAB staining. Results The expression of c-fos protein could be found in different regions of gerbil hippocampus following transient HBOC. The expression of e-fos protein was the highest in CA1 regain, and the expression decreased in CA2 , CA4 , CA3 regains in order. Conclusions Hippocampus is one of the neuronal localization of HBOC, and it is most infected in CA regain by HBOC.

关 键 词：惊厥 海马 神经元 c—fos蛋白 

分 类 号：R686[医药卫生—骨科学]