沙田柚配子体自交不亲和花柱消减文库的构建及分析  

Construction and Analysis of Suppression Subtractive Hybridization Library Related to Gametophytic Self-incompatibility in Style of Citrus grandis var. shatinyu Hort

作　　者：秦新民[1] 熊军[1] 阳晶[1] 万珊[1] 韦素玲[1] 

机构地区：[1]广西师范大学生命科学学院,广西桂林541004

出　　处：《热带亚热带植物学报》2008年第5期425-429,共5页Journal of Tropical and Subtropical Botany

基　　金：国家自然科学基金项目(30160007);广西研究生教育创新计划项目(2006106020710M33)资助

摘　　要：分别以沙田柚自交花柱cDNA为tester,异交花柱cDNA为driver,利用抑制性消减杂交技术构建了消减文库,文库的重组率高于95%,插入片段集中在100～500bp之间,对文库部分克隆进行测序并与GenBank中的同源序列进行比较,发现了一些类似于S1、S9-RNase、激酶类等与自交不亲和相关的基因。A suppression subtractive hybridization library of Citrus grandis var. shatinyu Hort was constructed by using the cDNA of self-pollinated styles as the tester and cross-pollinated styles as the driver. In the constructed library, the rate of recombination was more than 95% and the size of inserts ranged from 100 bp to 500 bp. After the partial clones of the library were sequenced and compared with homologous sequences in GenBank Database, some genes related with self-incompatibility were obtained, such as S1, S9-RNase and kinase, etc.

关 键 词：沙田柚 自交不亲和 抑制性消减杂交 CDNA文库 

分 类 号：Q785[生物学—分子生物学]