Pear Blossom Blast Caused by Pseudomonas syringae pv. syringae in China  

作　　者：QIU Wen HUAI Yan XU Fu-shou XU Li-hui XIE Guan-lin LI Bin YU Shan-hong LIU Jun-ying 

机构地区：[1]Institute of Biotechnology, Zhejiang University, Hangzhou 310029, P.R.China [2]Zhejiang Provincial Agricultural Bureau, Hangzhou 310029, P.R.China [3]Hangzhou Plant Protection Station, Hangzhou 310020, P.R.China

出　　处：《Agricultural Sciences in China》2008年第9期1091-1096,共6页中国农业科学（英文版）

基　　金：the National High Technology Research and Development Program of China (2006AA10A211);National Natural Science Foundation of China (30671397);Hangzhou Agricultural Development Foundation,China (2007-2008)

摘　　要：This study was done to determine the causal organism of the pear blossom and bud blast in China. It was identified by a bacteriological test, electro-microscopic observation, Koch＇s postulate test, Biolog, fatty acid methyl esters （FAMEs）, and a polymerase chain reaction （PCR） test, and compared with the standard reference strains. Six representative strains out of 20 pathogenic bacterial isolates from 16 diseased samples showed characteristics similar to three standard strains of Pseudomonas syringae pv. syringae from Belgium. They were identified as P. syringae pv. syringae with a Biolog similarity of 0.57-0.86 and FAMEs similarity of 0.58-0.81. The bacterium was reisolated from the symptomatic plants and blossoms. Identification as P. syringae pv. syringae was confirmed by using PCR primers and sequence tests, and compared with the above-mentioned results. The data supported the fact that the pear blossom and bud blast in China could be caused by P. syringae pv. syringae.This study was done to determine the causal organism of the pear blossom and bud blast in China. It was identified by a bacteriological test, electro-microscopic observation, Koch＇s postulate test, Biolog, fatty acid methyl esters （FAMEs）, and a polymerase chain reaction （PCR） test, and compared with the standard reference strains. Six representative strains out of 20 pathogenic bacterial isolates from 16 diseased samples showed characteristics similar to three standard strains of Pseudomonas syringae pv. syringae from Belgium. They were identified as P. syringae pv. syringae with a Biolog similarity of 0.57-0.86 and FAMEs similarity of 0.58-0.81. The bacterium was reisolated from the symptomatic plants and blossoms. Identification as P. syringae pv. syringae was confirmed by using PCR primers and sequence tests, and compared with the above-mentioned results. The data supported the fact that the pear blossom and bud blast in China could be caused by P. syringae pv. syringae.

关 键 词：pear blossom blast pear bud blast Pseudomonas syringae pv. syringae confirmation 

分 类 号：S436.612[农业科学—农业昆虫与害虫防治]