血吸虫卵及环丙沙星对小鼠TNBS肠炎的预防作用  

Protective effect of schistosome ova and ciprofloxacin in a model of TNBS-induced colitis of mice

作　　者：赵媛[1] 张顺财[1] 江捷[1] 邹峻[1] 刘红春[1] 

机构地区：[1]复旦大学附属中山医院消化内科,上海200032

出　　处：《复旦学报（医学版）》2008年第5期692-696,共5页Fudan University Journal of Medical Sciences

摘　　要：目的研究腹腔注射血吸虫卵及口服环丙沙星对TNBS肠炎小鼠肠道炎症及Toll样受体4(Toll-likereceptor 4,TLR4)的影响。方法Balb/c小鼠50只随机分为正常对照组(10只)、TNBS肠炎组(20只)、血吸虫卵组(10只)及环丙沙星组(10只)。血吸虫卵组于造模前第3、14天腹腔注射冰冻灭活血吸虫卵104个,环丙沙星组于制作肠炎模型前予小鼠口服环丙沙星50 mg.kg-1.d-1共2周。分别评价小鼠一般情况、死亡率、肠壁炎症程度、肠壁TLR4蛋白表达、肠壁tlr4 mRNA表达及血清TNF-α水平。结果血吸虫卵组小鼠死亡率较TNBS肠炎组明显降低(20%vs70%,P<0.05),肠壁炎症明显改善(Ameho criteria评分1.43±0.52vs4.21±0.61,P<0.01),肠壁TLR4表达下调(0.33±0.03vs0.76±0.05,P<0.01);血清TNF-α表达下调,但尚无统计学显著意义(29.62±9.71vs40.50±12.48,P>0.05)。环丙沙星组小鼠死亡率较TNBS肠炎组亦明显降低(20%vs70%,P<0.05),肠壁炎症改善(Ameho criteria评分1.54±0.71vs4.21±0.61,P<0.05),肠壁TLR4表达下调(0.40±0.03vs0.76±0.05,P<0.01),血清TNF-α表达下调,但尚无统计学显著意义(27.85±16.17vs40.50±12.48,P>0.05)。结论血吸虫卵及环丙沙星可有效预防TNBS诱导的小鼠肠炎,其作用机制可能与调节Th1/Th2平衡及下调TLR4表达有关。Objective To evaluate the effect of schistosome ova and ciprofloxacin on TNBS-induced colitis and the alteration of TLR4 in mice. Methods Fifty Balb/c mice were randomly divided into four groups： normal controls （n = 10）, TNBS colitis group （n = 20）, sehistosome ova group （n = 10） and ciprofloxacin group （n = 10）. Mice of schistosome ova group were exposed to 10 000 freeze-killed schistosome ova by intra-peritoneal injection 3 and 14 clays before TNBS treating. Mice of ciprofloxacin group were treated with ciprofloxacin （50 mg·kg^-1·d^-1） by oral administration. The following valuables were evaluated： common appearance, mortality, colon pathological changes, TLR4 and tlr4 rnRNA expression in colon and expression of TNF-α in serum. Results Compared with TNBS colitis group, the mortality of schistosome ova group was lower （20% vs 70%, P〈0.05）, the colonic inflammation was relieved（Ameho criteria scoring 1. 43±0. 52 vs 4. 21±0. 61, P〈 0. 01 ）, the expression of TLR4 was down-regulated （0.33±0.03 vs 0.76±0.05, P〈0.01 ） and expression of serum TNF-α was down-regulated but with no statistical significance （29.62±9.71 vs 40.50±12.48, P〉0. 05）. In the ciprofloxacin group, the mortality was lower when compared with TNBS colitis group （20% vs 70%, P〈0.05）, the colonic inflammation was relieved （Ameho criteria scoring 1.54±0.71 vs 4.21±0.61, P〈0. 05）. The TLR4 expression in the colon was down-regulated （0.40±0.03 vs 0. 76±0.05, P〈0. 01） and the serum TNF-aexpression was down-regulated but with no statistical significance （27.85±16.17 vs 40.50±12.48, P〉0. 05）. Conclusions Schistosome ova and ciprofloxacin can obviously prevent the TNBS-induced colitis in mice, which were supposed to due to the regulation of Th1/Th2 balance and the down-regulation of TLR4.

关 键 词：炎症性肠病 血吸虫卵 环丙沙星 TNBS肠炎 TOLL样受体4 

分 类 号：R516.1[医药卫生—内科学]