GLP-1(7-36)及其拟似物Exendin-4阻断高血糖诱导的胰腺血流重分布  

GLP-1 and Exendin-4 prevent the re-distribution of pancreatic islet perfusion stimulated by glucose administration

作　　者：吴琳[1] 黄镇[2] Ake Sjoeholm 高鑫[3] 

机构地区：[1]复旦大学附属中山医院老年病科,上海200032 [2]瑞典卡罗琳斯卡医学院附属南方医院内科,瑞典斯德哥尔摩11883 [3]复旦大学附属中山医院内分泌科,上海200032

出　　处：《复旦学报（医学版）》2008年第5期720-723,728,共5页Fudan University Journal of Medical Sciences

摘　　要：目的测定GLP-1和Exendin-4对大鼠胰岛微循环的影响。方法大鼠随机分为生理盐水组(NS)和葡萄糖组(GLU);每组再分为3个亚组,分别为对照组、GLP-1组和Exendin-4组。采用微球技术测定大鼠胰腺血流(pancreatic blood flow,PBF)和胰岛血流(islet blood flow,IBF)。用ELISA法测定血清胰岛素。结果GLP-1和Exendin-4不影响基础胰岛微循环,但降低糖负荷后IBF/PBF比值(GLP-1组为11.47%±1.11%vs14.33%±0.53%;Exendin-4组为11.25%±1.26%vs14.33%±0.53%,P<0.05),阻断高血糖诱导的胰腺血流向胰岛内重分布。GLP-1对基础和糖负荷后血糖无影响,Exendin-4显著降低基础血糖[(4.1±0.23)mmol/Lvs(5.4±0.37)mmol/L,P<0.05]和糖负荷后血糖[(17.9±0.97)mmol/Lvs(22.0±0.69)mmol/L,P<0.05]。结论GLP-1和其长效拟似物Exendin-4能有效调节糖负荷后的胰岛微循环。Objective To evaluate the effects of GLP-1 and Exendin-1 on islet microcirculation in rats. Methods Adult male Wistar rats were randomly divided into two groups injected with either saline or glucose. Each group was further divided into 3 subgroups administered i. v with either GLP-1 , Exendin-4 or saline respectively. A microsphere technique was adopted to measure the pancreatic blood flow （PBF） and pancreatic islet blood flow （IBF）. Results Neither GLP-1 nor Exendin-4 affected basal islet microcirculation, however, both of them significantly decreased fraction IBF out of PBF [（11.47±1. 11）% vs （14. 33 ± 0. 53）% for GLP-1;（11. 25 ± 1. 26）% vs （14. 33 ±0. 53）% for Exendin-4, P〈0. 05 respectively] in hyperglycemic rats, thus prevented the glucose induced blood flow redistribution in favor of islet. GLP-1 did not affect blood glucose in either basal state or after intravenous glucose load, while intravenous injection of Exendin-4 significantly decreased both basal blood glucose level [（4. 1 ± 0. 23） mmol/L vs （5.4 ± 0. 37）mmol/L,P〈0. 05] and post-load blood glucose level [（ 17.9±0.97）mmol/L vs （22.0 ± 0.69）mmool/L, P〈0.05]. Conclusions GLP-1 and its long acting analogue Exednin-4 modulate pancreatic islet microcirculation after intravenous glucose administration.

关 键 词：GLP-1 EXENDIN-4 胰岛微循环 微球技术 

分 类 号：R335.6[医药卫生—人体生理学]