宫内给药对大鼠细菌性宫内感染胎盘IL-6与MMP-9表达的影响  

Intraamniotic administration on placental IL-6 and MMP-9 expressions in rats with intrauterine infection of E. coli

作　　者：刘建[1] 程浩[1] 邹姝丽[1] 兰易[1] 甘小玲[1] 

机构地区：[1]重庆医科大学附属第二医院妇产科,重庆400010

出　　处：《第三军医大学学报》2008年第19期1803-1806,共4页Journal of Third Military Medical University

基　　金：重庆市自然科学基金(CSTC,2005BB5256)~~

摘　　要：目的探讨头孢曲松钠羊膜腔内和静脉给药对大肠埃希菌所致宫内感染的晚孕SD大鼠胎盘组织白细胞介素-6(IL-6)及基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)表达的影响。方法妊娠15d的SD孕鼠36只,完全随机分成4组,每组9只。①妊娠第15天,所有孕鼠一侧宫角行1010/L大肠埃希菌菌液0.2ml接种。②妊娠第16天,羊膜腔内给药组(IUA)和对照组(IUC)分别于接种侧各羊膜腔内注射头孢曲松钠和等体积的生理盐水,静脉给药组(IVA)和对照组(IVC)母鼠尾静脉分别注射头孢曲松钠和等体积的生理盐水。③4组孕鼠自妊娠第17天起,每组每天随机处死3只,随机抽取其接种侧宫角顶端3个胎囊之一的胎盘行IL-6、MMP-9免疫组化染色后行半定量分析。结果胎盘IL-6和MMP-9表达水平在给药后第1、2、3天,各给药组低于各相应对照组(P<0.05),IUA组低于IVA组(P<0.05),IUC组高于IVC组(P<0.05)。IUA组和IVA组IL-6水平于给药后第2天最低(P<0.05),IUC组和IVC组IL-6表达水平及各组MMP-9表达水平逐日增高(P<0.05)。结论单次头孢曲松钠给药治疗晚孕SD大鼠大肠埃希菌宫内感染,羊膜腔内给药较母鼠静脉途径更有效,其可通过抑制IL-6和MMP-9在胎盘组织中的表达水平,降低炎症反应水平和胎膜早破的风险。Objective To explore the effect of ceftriaxone sodium administered intraamniotically and by maternal intravenous injection on the expressions of placental interleukin-6 （IL-6） and matrix metalloproteinase- 9 （MMP-9） in treatment of intrauterine infection with E. coli in late pregnant SD rats. Methods Thirty-six SD rats on gestation day 15 were randomly divided into four groups （9 in each）. On gestation day 15, 0. 2 ml E. coli suspension at a dose of 10^10/L was inoculated into one horn of uterus; On gestation day 16, each gestational sac in inoculated side of rats in intrauterine administration （IUA） group and control （IUC） group was given ceftriaxone sodium or appropriate volume of isotonic Na chloride （NS） intraamniotically, and each rat in maternal intravenous injection （IVA） group and control （IVC） group was given ceftriaxone sodium or appropriate volume of isotonic NS intravenously. From the gestation day 17 on, 3 rats of each group were sacrificed randomly each day. One of the top 3 sacs in inoculated side of each rat was randomly selected, and the expressions of IL-6 and MMP-9 were analyzed semi-quantitively by immunohistochcmical method. Results After administration, the levels of IL-6 and MMP-9 in each treatment group were lower than those in the corresponding control groups （P 〈 0.05 ）, and those in IUA group were lower than those in IVA group （P 〈 0.05 ）, but those in IUC group were higher than those in IVC group （P 〈 0.05）. The levels of IL-6 decreased to the lowest on the 2nd day after administration in both treatment groups （P 〈 0. 05 ）, but increased day by day in both control groups, while MMP-9 expressions increased gradually in each group （P 〈 0.05）. Conclusion Compared with maternal intravenous injection, ceftriaxone sodium administered intraamniotically with a single dose is more effective treatment for intrauterine infection of E. coli in late pregnant SD rats, through inhibiting the expressions of IL- 6 and MMP-9 in placenta and r

关 键 词：绒毛膜羊膜炎 大肠杆菌 妊娠末期 投药 羊膜腔内 白细胞介素-6 基质金属蛋白酶9 

分 类 号：R392.11[医药卫生—免疫学] R714.5[医药卫生—基础医学]