水稻无花粉型雄性不育基因的遗传关系剖析及定位  

Genetic Relationships Dissection and Molecular Mapping of a Non-pollen Male Sterility Gene in Rice

作　　者：蔡之军[1] 姚海根[1] 姚坚[1] 叶立华[1] 黄荣华[2] 杨仁崔[2] 

机构地区：[1]浙江省嘉兴市农业科学研究院,嘉兴314016 [2]福建农林大学作物遗传育种研究所,福州350002

出　　处：《分子植物育种》2008年第5期837-842,共6页Molecular Plant Breeding

基　　金：福建省教育厅项目(K02059)资助

摘　　要：本文以同时携带ms基因(无花粉型雄性不育)和wx(糯性)基因的wxBms系及cms、ms和wx基因的wxAms为材料,观察ms、cms及wx的遗传关系并对ms基因进行染色体定位。研究结果如下:遗传分析表明,无花粉型雄性不育受一对隐性核基因控制;糯性基因wx与ms表现独立遗传,非糯性突变导致ms后代育性异常分离,故只要去除保持系中带有ms突变基因的植株,便可实现不育系cms-龙特浦wxA的保纯繁殖;本试验中出现的cms-龙特浦wxAms/龙特浦wxB的F1代育性恢复现象解释为:ms基因上位cms,ms的雄性遗传表达早于cms,cms雄性不育的发育表达因滞后而被掩盖;采用SSR分子标记技术,将ms基因定位在第1条染色体上的SSR标记RM579和RM23,为克隆该基因进行雄性不育机理研究奠定基础。In this paper, the material, carrying ms （a genic no-pollen male sterility ） gene and wx （waxy） gene in rice, obtained from waxy hybrid rice A/B lines during developing waxy hybrid rice was used to explore the genetic relationships among ms, cms and wx. Furthermore, ms gene was mapped by SSR marker. The main results were submitted as follows： Genetic analysis suggested that ms was a recessive nuclear gene; The genetic technique for development of cms-Longtepu wxA breeder seeds was only to make ms gene free in the Longtepu wxB parent population. That F1 from a cross between cmsLongtepu wxA^ms and Longtepu wxB was fertile could be interpreted as fol- lows： ms gene was epistatical to cms, the genetic development of ms gene is superior to that of cms, which lead to the development of male sterility to be covered. RM579 and RM23 marker were found being linked with ms on Chromosome 1 with a distance of 4.2 cM and 3.8 cM respectively by application SSR marker, building a foundation for cloning of ms gene and further study on the mechanism of male sterility.

关 键 词：水稻 雄性核不育基因 遗传分析 分子标记 

分 类 号：S511[农业科学—作物学]