盐胁迫下虎尾草上调基因的筛选和鉴定  

Screening and Analysis of the Genes Whose Expressions are Up-regulated by Abiotic Stresses in Chloris virgata

作　　者：梁涵[1] 张欣欣[1] 管清杰[1] 罗秋香[1] 柳参奎[1] 

机构地区：[1]东北林业大学盐碱地生物资源环境研究中心,哈尔滨150040

出　　处：《分子植物育种》2008年第5期886-892,共7页Molecular Plant Breeding

基　　金：国家863计划(2002AA241111);黑龙江省杰出青年基金(JC200609)资助

摘　　要：虎尾草(Chloris virgata)是禾本科虎尾草亚族的一种一年生草本植物,适应性极强,能在恶劣的盐碱化土壤上生长。本实验构建了虎尾草的cDNA基因文库,随机挑选出2300个克隆,并且通过点杂交分析在NaCl胁迫下这些基因的表达情况。结果表明有59个基因在NaCl胁迫下强烈表达,这些上调基因中23个同时也被NaHCO3胁迫表达。这个结果暗示了NaCl逆境与NaHCO3逆境在基因表达的应答机制存在明显的差异。59个基因可以分成8类:能量代谢(21.7%),信号转导(5.0%),氧化逆境(13.3%),光合作用(10.0%),生长发育(5.5%),转录因子(1.67%),各种酶类(10.0%)和一些未知基因(28.3%)。从中选出了一些上调基因进行Northern杂交分析,Northern杂交表明这些基因中的有些是在根或叶中表达,与点杂交的结果有一致的趋势。Chloris virgata （feather fingergrass）, is an one-year-old herbaceous plant of Gramineae. It has high adaptability and can survive in highly saline soils. We randomly selected 2 300 clones from a C. virgata cDNA library and examined their expressions under NaC1 stress by Dot blot analysis. About 59 EST clones were strongly expressed under NaCl stress. Twenty-three of these up-regulated genes was also induced by NaHCO3. This result suggests that it has notable differences in response mechanism of gene expression under NaCl and NaHCO3. The ESTs were classified into eight groups： energy metabolism （21.7%）, signal transduction （5.0%）, oxidation adversity （13.3%）, photosynthesis （10.0%） and growth development represented （5.5%）, transcription factors （1.67%）, enzymes （10.0%） and unknown genes （28.3%）. Some up-regulated genes were selected for Northern blot. The Northern blot proved that some up-regulated genes expressed in roots, some in leaves, and others in both of them. The result was the same with Dot blot.

关 键 词：虎尾草 ESTS 基因表达 胁迫 上调 

分 类 号：Q943[生物学—植物学]