33个小麦品种(系)抗叶锈基因Lr19分子检测  

Molecular Marker Detection of Leaf Rust Resistance Gene Lr19 in 33 Common Wheat Cultivars

作　　者：陈云芳[1] 刘莉[1] 杨文香[1] 刘大群[1] 

机构地区：[1]河北省农作物病虫害生物防治工程技术研究中心,河北农业大学

出　　处：《分子植物育种》2008年第5期1015-1018,共4页Molecular Plant Breeding

摘　　要：由小麦叶锈菌(Puccinia triticinia)引起的小麦叶锈病在世界各地产麦区均有发生。利用抗病品种是防治该病害最经济、安全、有效的方法。小麦抗叶锈病基因Lr19是一个十分有效的抗叶锈性基因,自1966年首次将该基因从长穗偃麦草(Agropyron elongatum)转到普通小麦中,至今仍是一个应用潜力很大的抗病基因。本研究利用以PCR为基础的STS技术对以春小麦Thatcher为背景的50个近等基因系材料和TcLr19与Thatcher杂交F2小麦进行检测,并对33个小麦品种进行分子标记分析鉴定,结果如下:(1)对以春小麦Thatcher为背景的50个近等基因系材料和TcLr19×ThatcherF2代小麦进行PCR-STS检测,扩增结果表明在50个近等基因系材料中仅有TcLr19中出现一条130bp的DNA条带,STSLr19130标记在其他49个近等基因系材料中未检测到Lr19基因;F2代中表现感病的植株没有130bp的DNA片段,表现抗病的植株有130bp的DNA条带;重复两次结果相同,进一步证明了与小麦抗叶锈基因Lr19共分离的STS标记的稳定可靠。(2)利用以PCR为基础的STSLr19130标记对33个小麦品种的叶锈抗病基因进行分子分析,证明其中早优504、平原50、陕611小麦品种含有Lr19基因,为培育抗病品种提供了信息资源。Leaf rust caused by Puccinia triticinia is a very serious and the most widely distributed disease of wheat. Using resistant cultivars is an economic and environmental-friendly way for minimizing the losses caused by the disease. As an important leaf rust resistance gene, the Lr19 was firstly transferred into the wheat genome from Thinopyrum sp. in the form of the substitution line ＇Agrus＇ in 1966. At present, Lrl9 was great potential to be used for wheat production. A set of Thatcher near-isogenic lines of wheat cv. Thatcher containing in total 50 different Lr genes, F2 plants derived from a cross of TcLr19 with Thatcher and 33 wheat cultivars in＇China were used to examine STS （sequence tagged site） linked to lesfrust resistance gene Lr19. The main results were as follows： （1） STS marker for resistance gene Lrl9 against Puccinia triticinia was used to screen a set of Thatcher near-isogenic lines of wheat cv. Thatcher containing in total 50 different Lr genes. The STS marker linked to resistance gene Lrl9 was found to be reliable in diverse genetic backgrounds. The amplification product of the Lr19 gene STSLr19130 marker was not detected in the susceptible cv. Thatcher and in all of the near-isogenic lines examined except Lr19. STS analysis carried out in F2 plants derived from a cross of TcLr19 with Thatcher was shown that the 130 bp bind appeared in F2 plant conferring resistance against leaf rust as well as in TcLr19, but not in the susceptible F2 plant. It proved the STS markers are reliable for Lr19 detection. （2） STS analysis carried out in 33 wheat cultivars, the result showed that the 130 bp bind appeared in PCR product of three cultivars including Pmgyuan50, Zaoyou504, Shan611.

关 键 词：小麦 抗叶锈基因LrJ9 分子检测 STS 

分 类 号：S512.1[农业科学—作物学]