新型微波天线热凝大鼠坐骨神经的实验研究  

Experimental study of rat sciatic coagulation with a novel microwave antenna

作　　者：何金霞[1] 张俊德[1] 朱伟良[1] 李虹义[1] 张积仁[1] 

机构地区：[1]南方医科大学珠江医院肿瘤中心,广州510282

出　　处：《中华物理医学与康复杂志》2008年第8期523-527,共5页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的观察新型微波天线热凝sD大鼠坐骨神经后，神经形态和功能的长期连续变化。方法75只SD大鼠随机分成A、B、C组，分别以10w×6S、20W×6S、30W×6s的功率时间组合热凝其坐骨神经，于热凝后0，2，7，30，60d进行电生理检测和超微结构观察。于热凝后7，30，60d检测其静态坐骨神经功能指数。结果B、C两组热凝后0，2，7，30d时的静态坐骨神经功能指数、神经传导速度和复合动作电位最大幅值都有下降，与热凝前比较差异有统计学意义（P〈O．05），但B组于60d后恢复接近热凝前，而c组未见恢复。A组热凝后超微结构改变轻微，B组热凝后髓鞘疏松，进而崩解变性坏死，但在60d后神经纤维再生修复。c组热凝后髓鞘崩解断裂或凝固坏死、轴突坏死、基底膜消失，60d后大量胶原纤维生成阻碍神经纤维再生。结论微波热凝神经能导致神经传导功能的下降甚至消失，通过热凝毁损神经而缓解疼痛，微波热凝神经止痛可作为一种新的疼痛治疗手段。Objective To observe the sequential uhrastruetural and eleetrophysiologieal changes in the sci- atic nerve coagulated by a newly-designed microwave antenna. Methods A total of 75 Sprague-Dawley rats were randomly divided into groups A, B and C and irradiated with microwaves at 10, 20 or 30 Watts, for 6 seconds to co- agulate the left sciatic nerve. Electrophysiological effects and sequential uhrastruetural changes were observed on the 0th, 2nd, 7th, 30th and 60th days after coagulation. A static sciatic index was calculated based on measurements of the footprint on the 7th, 30thand 60th days after coagulation. Results On the 0th,2nd,7th and 30th days after＇co- agulation, the static sciatic index, the nerve conduction velocity and the amplitude of the action potentials ih groups B and C had decreased significantly compared with those before coagulation. On the 60th day after coagulation, signifi- cant recovery was observed in groups A and B, but not in group C. Only mild aheration in uhrastructure was found, and only in group A. The prominent changes in ultrastructure in group B included broken Schwann cell membranes and myelin disintegration. There were severe injuries in group C, including myelin disintegration, cell deformity, co- agulative necrosis, axon necrosis, basement membrane necrosis and demyelination. The structure of the sciatic nerve in group B had partially recovered after 60 days, but group C showed no recovery at all. Conclusion Microwave coagulation of a nerve can block its conduction, and even destroy the nerve. Percutaneous microwave coagulation is clinically feasible and can be an alternative treatment for pain.

关 键 词：微波 坐骨神经 神经电生理 

分 类 号：R651.3[医药卫生—外科学]