双氢青蒿素对卵巢上皮性癌细胞体外增殖及有丝分裂原激活的蛋白激酶磷酸化水平的影响  

Effects of dihydroartiminisin on proliferation and phosphorylation of mitogen-activated protein kinase in epithelial ovarian cancer cell lines

作　　者：谭先杰[1] Jean Plouet 郎景和[1] 吴鸣[1] 沈铿[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院妇产科,100730 [2]Institut National de laSante et de la Medicale Unit6 689

出　　处：《中华妇产科杂志》2008年第9期662-665,共4页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金（30100199）;北京科技新星计划（2003325）

摘　　要：目的探讨双氢青蒿素对卵巢上皮性癌（卵巢癌）细胞体外增殖的影响，以及对卵巢癌细胞有丝分裂原激活的蛋白激酶（MAPK）磷酸化水平的影响。方法双氢青蒿素处理卵巢癌细胞株SKOV3和OVCAR3细胞后，四甲基偶氮唑蓝比色法检测SKOV3和OVCAR3细胞的体外增殖抑制效应，蛋白印迹法检测SKOV3和OVCAR3细胞中两种MAPK[即细胞外信号调节激酶（ERK）1／2和038蛋白激酶]的磷酸化水平，以未用双氢青蒿素处理的SKOV3和OVCAR3细胞作为对照。结果双氢青蒿素处理72h后，SKOV3和OVCAR3细胞的50％抑制浓度（Ic。）平均分别为（9．0±1．4）和（5．5±1．2）i^mol／L。双氢青蒿素处理后，SKOV3和OVCAR3细胞中ERK1／2的磷酸化水平明显降低，相对于对照细胞分别下降了64．2％和75．3％，差异均有统计学意义（P〈0．05）；而p38蛋白激酶的磷酸化水平无明显变化，相对于对照细胞仅分别下降了8．5％和6．4％，差异均无统计学意义（P〉0．05）。结论双氢青蒿素能制卵巢癌细胞的体外增殖，该作用可能与其下调癌细胞内ERK1／2的磷酸化水平有关。Objective To determine the effect of dihydroartiminisin on the proliferation and phosphorylation of mitogen-activated protein kinase （MAPK） in SKOV3 and OVCAR3 ovarian cancer cell lines. Methods Methyl thiazolyl tetrazolium assay was performed to evaluate the anti-proliferative effect of dihydroartiminisin in SKOV3 and OVCAR3 cells, and Western blot was used to determine its effect on phosphorylation level of MAPK, including extra-cell regulated kinase （ERK） 1/2 and p38 protein kinase, in the two cell lines. Resalts Dihydroartiminisin inhibited the proliferation of ovarian cancer cells in vitro, with a mean of 50% inhibition concentration （ ICso ） at 72 h of （9.0 ± 1.4） Is, mol/L for SKOV3 and（5. 5 ± 1.2） μmol/L for OVCAR3 respectively. Compared to cells without dihydroartiminisin treatment, phesphorylation level of ERK 1/2 in SKOV3 and OVCAR3 cells treated with dihydroartiminisin decreased by 64. 2% and 751 3% respectively （ P 〈 O. 05 ）, while phosphorylation of p38 protein kinase in SKOV3 and OVCAR3 only decreased by 8.5% and 6. 4% respectively （P 〉 0.05）. Conclusion Dihydroartiminisin can inhibit the proliferation of ovarian cancer cell in vitro, probably through down-regulation of the phosphorylation of ERK 1/2 in ovarian cancer cells.

关 键 词：卵巢肿瘤 肿瘤细胞 培养的 细胞增殖 青蒿素类 丝裂原激活蛋白激酶类 磷酰化 

分 类 号：R686[医药卫生—骨科学]