人体正中神经内部显微结构的三维重建与可视化研究  

Three-dimensional reconstruction and visualization of internal microstructure of median nerve

作　　者：孙廓[1] 胡平[2] 张峰 张键[1] 李华[2] 陈增淦[1] 李智[1] 陈统一[1] 陈中伟[1] 林宗楷[2] 

机构地区：[1]复旦大学附属中山医院骨科,上海200032 [2]中国科学院计算技术研究所智能中心,北京100080 [3]Division of PlasticSurgery,University of Mississipi Medical Center

出　　处：《中国矫形外科杂志》2008年第18期1385-1388,共4页Orthopedic Journal of China

基　　金：国家自然科学基金项目(项目编号:30471744)

摘　　要：[目的]探索应用计算机处理人体正中神经全长连续冰冻组织切片的二维图像信息,开发3DNerve神经三维可视化系统,重建人体正中神经内部显微结构并实现三维可视化。[方法]取新鲜人尸正中神经标本1例,以人长发为定位线、OCT包埋剂包埋、采用连续冰冻组织切片、乙酰胆碱脂酶组织化学法染色、高分辨率扫描仪获取二维数码信息后、应用3DNerve对正中神经显微结构进行三维重建。[结果]正中神经在不同断面神经束的数目、位置以及神经束内神经纤维的性质变化较大。连续断面观察显示各神经束均是混合束,未见有纯粹的感觉束或运动束的出现。通过3DNerve可在任意断面放大的视野下观察正中神经的显微结构,追踪各神经束在正中神经内的立体行径。从而动态地展示正中神经内部神经束的复杂结构。[结论]重建的正中神经三维可视化真实地再现正中神经干全长及其内部各神经束和束组的三维立体结构,可为临床修复正中神经损伤提供精确的断层解剖图像。[ Objective ] To explore the application of computer in dealing with two-dimensional image information of human median nerve series freezing tissue sections, in order to develop nerve three-dimensional visualization system （3D Nerve） , and finally to reconstruct 3D internal microstructure of human median nerve and realize 3D visualization. [ Method] One fresh cadaver median nerve was taken, located with human hair and embedded in OCT. Series freezing tissue sections were made and stained with ACHE histochemistry method, and 2D image information was obtained through high resolution scanner. Microstructure of median nerve was finally reconstructed with 3D Nerve. [ Result] Different cross sections of median nerve had quite different number and positions of fasciculi. In addition, characters of fasciculus＇s internal nerve fiber were also quite different. Scross sections observation showed that all fasciculi were mixed fasciculi. With the 3D Nerve, the microstructure of median nerve was able to be observed in magnifying visual field at any cross section, and the tracking of stereo course of fasciculi in median nerve became possible. [ Conclusion] Reconstructed 3D visualization can reveal the whole microstructure of median nerve and the three dimensional stereo-structure of fasciculi and fasciculus groups exactly and truly. It can provide exact topographic atlas and facilitate precise clixical repair of median nerve injury.

关 键 词：正中神经 三维重建 三维可视化 乙酰胆碱脂酶 

分 类 号：R651.3[医药卫生—外科学]