新型“内标”式双重荧光自组装膜的制备和DNA的界面传感  

Preparation of Novel Dual Fluorescent Self-assembled Multilayer Film Bearing a Fluorescent Internal Standard for Interfacial Sensing

作　　者：汪志芳[1] 孙向英[1] 刘辉[1] 刘斌[1] 

机构地区：[1]华侨大学材料科学与工程学院,泉州362021

出　　处：《高等学校化学学报》2008年第9期1747-1749,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20575023);福建省国际合作重点项目(批准号:2006I0021);福建省自然科学基金(批准号:D0710017);教育部留学回国人员科研启动基金;教育部现代分析科学重点实验室(厦门大学)资助

摘　　要：In this paper we described the construction of a novel dual fluorescent film sensor using fluorescent dye Acridine Orange(AO) as an internal standard and CdTe as a fluorescent probe.Poly-sodium-4-styrenesulfonate(PSS) and Chitosan(CS) were respectively assembled between AO and CdTe.The constructed self-assembly multilayers,Quartz/APES/PSS/AO/PSS/CS/CdTe,emits dual fluorescence at 600 and 530 nm,respectively.AO is shielded effectively by the assembled PSS and CS and thus its fluorescent intensity I1 doesn′t change with the variation of the analyte concentration,thereby acting as an internal standard to provide reference signal.The ratio of dual fluorescence intensities of the thus constructed film sensor is not affected by the variations of the external environment factors such as excitation intensity and sensor position in the cell holder.A good linear relationship was found between dual fluorescence intensity ratio and DNA concentration over 0-250 ng/L.Quartz/APES/PSS/AO/PSS/CS/CdTe film is capable of an extremely highly sensitive detection for DNA with a detection limit of 6.6 ng/L.The analysis based on dual fluorescent self-assembled multilayers bearing a fluorescent internal standard would greatly improve the detection accuracy.In this paper we described the construction of a novel dual fluorescent film sensor using fluorescent dye Acridine Orange（AO） as an internal standard and CdTe as a fluorescent probe. Poly-sodium-4-styrenesul fonate（PSS） and Chitosan （CS） were respectively assembled between AO and CdTe. The constructed self-assembly muhilayers, Quartz/APES/PSS/AO/PSS/CS/CdTe, emits dual fluorescence at 600 and 530 nm, respectively. AO is shielded effectively by the assembled PSS and CS and thus its fluorescent intensity I1 doesn＇t change with the variation of the analyte concentration, thereby acting as an internal standard to provide reference signal. The ratio of dual fluorescence intensities of the thus constructed film sensor is not affected by the variations of the external environment factors such as excitation intensity and sensor position in the cell holder. A good linear relationship was found between dual fluorescence intensity ratio and DNA concentration over 0--250 ng/L. Quartz/APES/PSS/AO/PSS/CS/CdTe film is capable of an extremely highly sensitive detection for DNA with a detection limit of 6.6 ng/L. The analysis based on dual fluorescent self-assembled multilayers bearing a fluorescent internal standard would greatly improve the detection accuracy.

关 键 词：荧光内标 双重荧光 自组装膜 界面传感 CDTE 吖啶橙 

分 类 号：O657[理学—分析化学] O647[理学—化学]