基于主成分分析和聚类分析的FTIR不同地理居群香果树多样性分化研究  

A Study of the Diversity of Different Geographical Populations of Emmenopterys Henryi Using FTIR Based on Principal Component Analysis and Cluster Analysis

作　　者：张志祥[1] 刘鹏[1] 康华靖[1] 廖承川 陈子林 徐根娣[1] 

机构地区：[1]浙江师范大学植物学实验室,浙江金华321004 [2]九龙山国家级自然保护区管理局,浙江遂昌323300 [3]大盘山国家级自然保护区管理局,浙江磐安322300

出　　处：《光谱学与光谱分析》2008年第9期2081-2086,共6页Spectroscopy and Spectral Analysis

基　　金：浙江省自然科学基金项目(399277);浙江省新苗人才计划项目(2007G60G2030026);金华市科技项目(2005-1-318)资助

摘　　要：应用傅里叶变换红外光谱（FTIR）法测定了浙江省7个不同地区香果树茎和叶的红外光谱,以721～3366cm^-1范围内的吸收峰吸光度为指标,以红外光谱图为对象,应用主成分分析（principal component analysis,PCA）和聚类分析（Cluster analysis）,比较了7个不同地理居群香果树茎和叶在红外光谱图上的差异程度,即各吸收峰所对应的化学成分含量差异。结果表明,香果树茎和叶的红外光谱存在一定差异,叶在聚类分析中所表现出来的居群间的距离系数和主成分分析三维排序图中各居群的相对位置都较大,在研究植物不同地理距离居群多样性分化方面具有比茎更好的效果。与其他地区地理距离相对较远的大盘山和古田山香果树居群红外光谱具有特殊性,多样性分化显著。九龙山、乌岩岭和松阳的香果树居群红外光谱图各有其特点,在三维排序图上的位置差异不大,聚类分析也显示这几个居群间距离系数很小,说明这几个地区香果树居群在植物化学组成上存在一定差异,但居群多样性分化不显著。发现基于FTIR的主成分分析排序图和聚类分析图能够在一定程度上表征植物不同地理居群间多样性的分化,这也表明,FTIR在应用于濒危植物保护方面具有广泛的应用前景。Emmenopterys henryi, an endemic species in China, has been one of the grade Ⅱ national key conservation rare and endangered plants. The spectra of stem and leaf of Emmenopterys henryi sampling from seven different geographical populations were determined by Fourier transform infrared （FTIR） spectrometry with OMNI-sampler directly, fast and accurately. A positioning technology of OMNIC E.S.P.5.1 intelligent software and ATR correction was used. It was scanned for the background before the determination of every example. The peak value and absorbance were ascertained using a method of baseline correction in infrared spectra. Based on the indices of wave number-absorbance from 721 to 3 366 cm-1, the differences of these infrared spectra were compared by the methods of principal component analysis （PCA） and cluster analysis. Results showed that there were some differences in FTIR spectra between stem and leaf of Emmenopterys henryi, so it was better to study the diversity of different geographical populations through using the leaf, for which the distance coefficient of clustering analysis plot and the position relationship of principal component analysis three-dimensional plot of the seven populations were bigger. Being far away from others populations, the infrared spectra of Emmenopterys henryi in Dapan Mountain and Gutian mountain had special characteristics, indicating significant diversity. At the same time, the infrared spectra of Jiulong Mountain, Wuyan Mountain and Songyang populations had their own characteristics. There were no significant difference in the position relationship of three-dimensional plot and distance coefficient of clustering analysis plot, showing that the chemical compositions of these three populations were of little difference, and the diversity differentiation was not remarkable. However, there were some significant differences in populations’ diversity between Fengyang Mountain and Wencheng. It was indicated that the chemical composition of Emmenopterys henryi was affecte

关 键 词：傅里叶变换红外光谱 主成分分析 聚类分析 地理距离 多样性分化 香果树 

分 类 号：O657.3[理学—分析化学]