HPLC法测定维生素C制剂  

Determination of Vitamin C Preparations by HPLC

作　　者：李文玉[1] 李虎将 

机构地区：[1]吉林省通化市食品药品检验所,通化134000 [2]通化市疾病预防控制中心

出　　处：《中国药事》2008年第9期810-812,共3页Chinese Pharmaceutical Affairs

摘　　要：对2005年版《中国药典》收载的维生素C片、维生素C注射液及维生素C丸含量测定方法进行改进,建立HPLC法测定维生素C含量。采用Kromasil C18色谱柱;以甲醇-0.1%磷酸（5∶95）为流动相;柱温：25℃;流速：0.8mL·min^-1;检测波长：242nm。维生素C进样量在0.0618～0.5562μg范围内呈良好的线性关系;平均回收率（n=6）为100.0%,RSD为0.33%。本方法简便、快速、结果准确,可作为维生素C多种制剂的质量控制标准。The methods for the determination of Vitamin C Tablets,Vitamin C Injection and Vitamin C Pills in ChP（2005） were improved.An HPLC method was developed for the determination of Vitamin C Tablets.The HPLC system consisted of C18 column,with methanol-0.1% phosphoric acid（5∶95） as mobile phase,at 25℃.The wavelength was 242nm.The flow rate was 0.8mL·min^-1 and the injection volume was 10μL.The linear ranger of vitamin C was 0.0618～0.5562μg.The average recovery for the compound was 100.0%（RSD=0.33%,n=6）.The method is simple,quick and accurate.It is suitable for quality control of many kinds of vitamin C preparation.

关 键 词：HPLC法 维生素C片 维生素C注射液 维生素C丸 维生素C 含量测定 

分 类 号：R927.2[医药卫生—药学]