牛珀至宝微丸对内毒素急性肺损伤水通道蛋白表达的影响  

Effect of Niupo Zhibao Pellets on Aquaporin Expression in Endotoxin-induced Acute Lung Injury

作　　者：黄洁 李慧[2] 付友金[2] 陈肖君[2] 黄玮[2] 杨丽娜[2] 陈阳燕[2] 张蓉[2] 叶兵[2] 杜少辉[2] 

机构地区：[1]深圳市人民医院中医科,广东深圳518020 [2]深圳市中医院内科,广东深圳518033

出　　处：《新中医》2008年第10期99-100,共2页New Chinese Medicine

摘　　要：目的:观察牛珀至宝微丸对内毒素急性肺损伤纤维化水通道蛋白表达的影响。方法:静脉注射内毒素(LPS)、腹腔注射D-氨基半乳糖(D-GalN)造成内毒素休克模型,用牛珀至宝微丸干预处理,HE染色观察肺损伤,Van Gieson法染色检测肺胶原纤维表达,免疫组化染色观察AQP-1和AQP-5表达。结果:内毒素造成严重的急性肺损伤,肺组织胶原纤维染色显著增强,AQP-1和AQP-5表达减弱;牛珀至宝微丸能减轻急性肺损伤及肺组织胶原纤维表达,并能提高AQP-1和AQP-5表达。结论:牛珀至宝微丸能减轻内毒素急性肺损伤和纤维化的机理可能与调节AQP-1和AQP-5表达相关。Objective:To observe the effect of Niupo Zhibao Pellets(NZP)on aquaporin expression in endutoxin-induced acute lung injury and fibrosis.Meth- ods:Intravenous injection of lipopolysaccharide(LPS)and intraperitoneal injection of D-galactosamine(D-GalN)were applied to induce the model.NZP was used for the pretreatment.After treatment,the lung injury was observed by HE staining,lung col- lagen fiber expression was detected by Van Gieson staining,and the expression of AQP-1 and AQP-5 was observed by immunohistochemicai method.Results:LPS induced severe acute lung injury,the expression of lung collagen fiber became stronger,and the expression of AQP-1 and AQP-5 became weak.NZP relieved the acute lung injury,decreased the expression of lung collagen fiber and increased the expression of AQP-1 and AQP-5.Conclusion:The mechanism of NZP in relieving endotoxin-induced acute lung injury and fibrosis is probably related with the regula- tion of AQP-1 and AQP-5 expression.

关 键 词：急性肺损伤 肺纤维化 牛珀至宝微丸 内毒素 水通道蛋白 

分 类 号：R259.638[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]