罗丹明6G的三维荧光和共振散射光谱  

Spectrophotometric Studies on Three-Fluorescence and Resonance Scattering of Rhodamine 6G

作　　者：夏国朝[1] 陶慧林[1] 

机构地区：[1]桂林工学院材料与化学工程系,广西桂林市建干路12号541004

出　　处：《光谱实验室》2008年第5期773-778,共6页Chinese Journal of Spectroscopy Laboratory

摘　　要：研究了罗丹明6G(R6G)的荧光光谱、共振散射光谱和吸收光谱,讨论了共振光散射与共振荧光的区别与联系。在罗丹明6G水溶液的三维荧光等高线光谱中,瑞利散射线与荧光等高线有部分相交。共振散射峰(544nm)介于荧光激发峰(530nm)和发射峰(552nm)之间。由光偏振实验,测得R6G共振散射光谱544nm处的偏振度P为0.0105。上述实验结果证明,R6G的共振散射峰主要是共振荧光。共振光散射信号随pH值增大而增强的机理是R6G酸碱平衡移动导致荧光型体的形成。由于自吸收的影响,荧光强度、共振散射光强度与R6G浓度之间不是严格的线性关系。Fluorescence,resonance light scattering（RLS） and absorption spectra of Rhodamine 6G（R6G） have been studied to discuss the difference and relationship between RLS and resonance fluorescence. In three-dimensional fluorescence contour spectra of R6G aqueous solutions, Rayleigh scattering line intersects with fluorescence contour. The RI.S peak （544nm） of R6G lies just between fluorescence excitation peak （530 nm）and emission peak （552nm）. In light polarization experiment, the polarization of RLS at 544nm was measured to be P=0. 0105. All the above experimental fact reveals that the RLS peak of R6G mainly consists of resonance fluorescence. The mechanism of RLS enhancement with the pH increase is because of the formation of fluorescence species in acid-base equilibrium of R6G solutions. Since light scattering is affected by light absorption,relationship between fluorescence intensity,RLS intensity and R6G concentration is not strictly linear.

关 键 词：罗丹明6G 三维荧光 共振光散射 共振荧光 光偏振 

分 类 号：O657.32[理学—分析化学]