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作 者:郑毅男[1]
出 处:《吉林农业大学学报》2008年第4期486-491,共6页Journal of Jilin Agricultural University
基 金:教育部博士点基金;吉林省科技发展重点项目(20040207-1-2)
摘 要:不同的提取方法对皂苷含量影响显著,热回流法与超声波法和微波法相比对人参皂苷的提取率最高,而且在加热过程中丙二酰基人参皂苷分别转化成相应的中性皂苷。对生长在吉林省的栽培参和林下参的6种主要皂苷(Rg1,Re,Rb1,Rc,Rb2,Rd)进行高效液相色谱(HPLC)分析,结果显示不同地区栽培的人参主根中皂苷含量存在显著差别,且人参皂苷Rg1和Re的化学型组成比例差异较大。吉林人参明显存在3种化学型,分别为高Rg1低Re化学型,低Rg1高Re化学型,Rg1、Re几乎相等化学型。人参不同种群、不同生长年限、不同栽培方式对人参皂苷含量及组成比例都会产生影响。The extraction methods significantly influenced ginsenosides content, and higher extraction ratio was obtained by heated reflux extract, supersonie and microwave methods due to the conversion of malonyl ginsenosides into ginsenosides correspondingly during heating. Determination and contents of six ginsenosides (Rg1, Re, Rb1, Re, Rb2, Rd) from the cultivated and similar wild ginseng, collected from Jilin province, were investigated by high performance liquid chromatography (HPLC). The results showed significant differences of ginsenoside contents and composition from different cultivated regions, and differenee of ratio ginsenoside Rg1 to Re were found. We thought there were three ehemotypes such as high Rg1/low Re, high Re/low Rgm and quasi equal type in Jilin ginseng. In addition, different populations, ages, and cultivation ways also affected the compositions and content ratio of ginsenosides.
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