新霉素人工抗原的合成与鉴定  

Synthesis and identification of neomycin artificial antigen

作　　者：张改平[1] 刘宣兵[1] 彩鸿翔[1] 邓瑞广[1] 游雷鸣[1] 赵东[1] 侯玉泽[2] 

机构地区：[1]河南省农业科学院动物免疫学重点实验室,郑州市450002 [2]河南科技大学食品与生物工程学院,郑州市450002

出　　处：《中国饲料》2008年第18期38-41,共4页China Feed

基　　金："十一五"国家科技支撑计划(2006BAK02A21)

摘　　要：用水溶性碳化二亚胺(EDC)法将新霉素(NEO)偶联于载体蛋白牛血清白蛋白(BSA)和鸡卵清蛋白(O-VA),合成免疫原BSA-NEO和包被原OVA-NEO,用红外扫描(IR)、SDS-PAGE法进行鉴定;用BSA-NEO免疫BALB/C小鼠,间接ELISA测定多抗上清(pAb)效价,阻断ELISA鉴定其敏感性,交叉反应试验鉴定其特异性。结果表明,BSA-NEO的红外光谱与BSA相比发生了改变,BSA-NEO的电泳条带与BSA的相比出现了明显的拖尾现象;通过系列ELISA鉴定,获得了高价、敏感、特异的pAb,为NEO残留免疫学检测方法的建立奠定了基础。Neomycin （NEO） was conjugated to carrier protein bovine serum albumin（BSA） and ovalbumin （OVA） by the method of carbodiimide （EDC）,and immunizing antigen BSA-NEO,the coating antigen OVA-NEO were obtained in the same way.IR and SDS-PAGE were used to identify NEO artificial antigen.BALB/C mice were immunized with BSA-NEO,the titre of polyclonal antibody（pAb）was detected by indirect ELISA ,the sensitivity of pAb was identified by blocking ELISA and the specificity of pAb was identified by the method of cross-reaction test.The results showed that IR spectrogram of BSA-NEO has been changed when compared to BSA,the electrophoresis strap of BSA-NEO appeared the visible trail,when compared to BSA,the high-titer,sensitive and specific anti-NEO pAb have been produced in sera of immunized BALB/C mice,which made it possible to establish immunoassay of NEO residues in feed and animal food.

关 键 词：新霉素 人工抗原 合成 鉴定 

分 类 号：S859.84[农业科学—临床兽医学]