小鼠胚胎冷冻和重复冷冻的研究  

The Study of Mouse Embryo Cryopreservation and Double Freezing

作　　者：冯勋伟[1] 王新庄[1] 李义书[1] 王轶敏[1] 牛晖 

机构地区：[1]西北农林科技大学动物医学学院 [2]河南省肉牛工程技术研究中心,河南郑州450003

出　　处：《西北农业学报》2008年第5期16-20,共5页Acta Agriculturae Boreali-occidentalis Sinica

摘　　要：研究了不同冷冻方法对小鼠胚胎的冷冻保护效果及能否进行重复冷冻、重复冷冻后胚胎的继续发育率情况。采用乙二醇(1.8 mol/L、2.7 mol/L、3.6 mol/L、4.5 mol/L)和EFS玻璃化液(EFS20、EFS30、EFS40)对小鼠的桑椹胚和囊胚进行程序化冷冻和细管法玻璃化冷冻并再进行重复冷冻。结果表明以1.8mol/L EG和EFS30对小鼠的胚胎冷冻效果最好,一次冷冻解冻后的发育率桑椹胚分别为92.5%和91.5%,囊胚分别为82.8%和81.3%,两种冷冻方法差异不显著(P>0.05)。经1.8 mol/L EG首次冷冻再经EFS30重复冷冻的效果最好,桑椹胚和囊胚的发育率分别为53.8%和33.9%。To explore the effects of different freezing procedure on mouse embryos and the development of after double frozen. Mouse morula and blastocyst were cryopreserv.ed using ethylene glycol （EG） （1.8 mol/L, 2.7 mol/L,3.6 mol/L,4.5 mol/L）and EFS （EFS20,EFS30.EFS40）as cryoprotective agent with slow-freezing , straw vitrification and double frozen. Results showed that when the mouse embryos were cryopreserved by the 1.8mol/LEG and EFS30, the effects are the best, after the first freezing and thawing , the development rates of morula are 92.50//00 and 91.5% , blastocyst are 82.8% and 81.3% （P）0.05）. After double freezing and thawing , the development rates of morula and blastocyst are 5：3.8% and 33.9%.

关 键 词：小鼠 胚胎冷冻 重复冷冻 桑椹胚 囊胚 

分 类 号：S814[农业科学—畜牧学]