BTH对黄瓜叶片H_2O_2生成和相关酶活性的影响  

Effects of BTH on the H_2O_2 Production and Related Enzyme Activities in Cucumber Leaves

作　　者：李玉红[1] 程智慧[1] 孟焕文[1] 杜慧芳[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《西北农业学报》2008年第5期298-301,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：西北农林科技大学"青年学术骨干支持计划"

摘　　要：以‘长春密刺’黄瓜幼苗为材料,对苯并噻二唑(BTH)处理或霜霉菌接种后黄瓜叶片的过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性与H2O2含量的变化进行了研究。结果表明,BTH处理或霜霉菌接种均可诱导叶片H2O2含量显著增加,BTH预处理后接种的叶片比接种叶片能更快地积累H2O2,H2O2参与了幼苗对霜霉病的抗性诱导;BTH处理后叶片SOD和POD活性升高,CAT活性受到一定程度的抑制。研究发现,H2O2含量的增加与SOD、POD活性升高、CAT活性下降有关;BTH通过调节H2O2的含量诱导黄瓜霜霉病抗性有关的防御基因表达是诱导抗性的机制之一。The activities of POD、SOD or CAT and H2O2 content were examined in cucumber leaves treated with BTH or inoculated with Pseudoperonospora cubensis f.sp.cucumerinum.Results showed H2O2 content in leaves treatment with BTH were increased significantly,and accumulated faster than control after inoculation.The result showed that H2O2 may be concerned with induced resistance against downy mildew.After treated with BTH,the activities of SOD or POD were enhanced while CAT activities were inhibited in leaves.The increase of H2O2 content may be related to the increase of SOD or POD activities and the decrease of CAT activities.That BTH induced defence gene expression through H2O2 was one of the main machinsm of induced resistance.

关 键 词：BTH 黄瓜霜霉病 H2O2 POD CAT SOD 

分 类 号：S642.2[农业科学—蔬菜学]