应用离心淘洗技术研究VP对PYM细胞毒性的影响  

STUDIES ON EFFECTS OF VERAPAMIL ON THE CYTOTOXICITY OF PINGYANGMYCIN BY CENTRIFUGAL ELUTRIATION

作　　者：何农高 

机构地区：[1]南充师范学院生物系

出　　处：《华西药学杂志》1990年第1期15-18,共4页West China Journal of Pharmaceutical Sciences

摘　　要：应用最新细胞同步化方法——离心淘洗技术,同步分离体外培养小鼠红白血病细胞(MELC),研究钙拮抗剂异博定(Verapamil:VP)对国产抗癌药物平阳霉素(Pingyangmycin:PYM)细胞毒性的影响。无毒剂量的VP使PYM对未同步化的MELC细胞的毒性加强;同步化后,VP的加入使PYM对G2M期细胞的毒性增强,而对G1期和S期细胞的毒性无明显改变。结果提示,VP与PYM的作用可能有相同的周期特异性。The effect of calci- um antagonist verapamil (VP) on the cytotoxicity of a domestic antitumor drug Pingyangmycin (PYM) was investigated in the cultured MELC cells synchrolized by the new cell synchrolization techni- que: Centrifugal Eluriation. In the asyn- chrolized cells, nontoxic-dosage VP enha- need the cytotoxicity of PYM drastically; In the synchrolized cells, VP enhanced the cytotoxicity of PYM to G_2M phase cells but not to G1 and S phase cells. The results suggest that the effect of VP may has a common cell cycle specifity with PYM.

关 键 词：异博定 平阳霉素 毒性 离心淘洗 

分 类 号：R965.3[医药卫生—药理学]