核因子-κB在大鼠烧伤后中性粒细胞致心功能损害中的作用  

Effects of NF-κB activation on pathogenesis of early myocardial dysfunction induced by PMN in burned rats

作　　者：李志清[1] 黄跃生[2] 杨宗城[2] 王甲汉[1] 

机构地区：[1]南方医科大学南方医院烧伤科,广州510515 [2]第三军医大学西南医院全军烧伤研究所、创伤、烧伤与复合伤国家重点实验室

出　　处：《中华急诊医学杂志》2008年第9期921-924,共4页Chinese Journal of Emergency Medicine

基　　金：国家重点基础研究发展规划基金资助项目（G1999054202）

摘　　要：目的探讨大鼠烧伤早期核因子-κB（NF-κB）活化对中性粒细胞（PMN）在心肌组织中聚集和发生损害作用的影响。方法将170只Wistar大鼠随机分正常对照组（20只）、烧伤组（90只）、烧伤后吡咯烷二硫代氨基甲酸盐干预组（PDTC组，60只）。后两组大鼠均于背部造成35％体表总面积Ⅲ度烧伤后，立即腹腔注射等渗盐水，且PDTC组同时皮下注射PDTC250mg／kg。对照组除不烧伤外，其余处理同烧伤组。伤后1、3、6、12、24h，凝胶电泳迁移率分析法检测心肌组织NF-κB活性；逆转录-聚合酶链式反应（RT-PCR）检测心肌组织白细胞介素8（IL-8）和细胞间黏附分子-1（ICAM-1）mRNA的表达；八导生理记录仪记录左心室最大收缩压（LVSP）、左心室舒张末压（LVEDP）及心室内压最大上升速率（＋dp／dtmax）和下降速率（-dp／dtmax）；并测定心肌组织髓过氧化物酶（MPO）活性。对照组作相同检测。数据采用SPSS10．0统计软件行单因素方差分析。结果烧伤组大鼠心肌组织NF-κB活性伤后迅速增高，伤后1h达峰值（20．27±3．43）×10^4积分灰度值（A），明显高于对照组[（2．18±0．38）×10^4A，P〈0．01]，伤后24h仍高于对照组（P〈0．01）。伤后3、6、12、24h心肌组织ICAM—1和IL-8mRNA表达、MPO活性均较对正常对照组明显升高（P〈0．01），LVSP、±dp／dtmax明显低于正常对照组（P〈0．01），而LVEDP高于正常对照组（P〈0．01）。与烧伤组比较，PDTC组上述指标指标均有明显的改善（P〈0．01）。结论严重烧伤可活化心肌组织NF-κB，从而上调黏附分子和趋化因子等的合成和释放，导致PMN在心肌组织中聚集，引起心肌功能损害。Objective To investigate the effects of NF-κB activation on pathogenesis of PMN aggregation in myocardium and early myocardial dysfunction induced by polymorphonuclear leukocyte （PMN） in burned rats. Method One hundred and seventy wistar rats were randomly divided into three groups, control group （ n = 20, with isotonic saline solution）, burn group （ n = 90, with isotonic saline solution after burn）, burn and pyrrolidine dithioncarbamate group （PDTC group, n = 60, with isotonic saline solution and 250 mg/kg PI）TC after burn） .The rats in burn group and PDTC group underwent 35% TBSA full-thickness burns on the back. The activaty of myocardial NF-κB was tested by electrophoretic mobility shift assay （EMSA） at 1, 3, 6, 12,24 postburn hours （PBH）. Expressions of myocardial IL-8 and ICAM-1 mRNA were assessed by reverse transcription polymerase chain reaction （RT-PCR） at 3,6,12,24 PBH. Meanwhile, the avtivity of myocardial myeloperoxidse（MPO）, the left ventricular systolic pressure（LVSP） and the left ventricular end diastolic pressure （LVEDP） as well as maximum positive and negative left ventricular pressure change （＋_ dp/dtmax） were observed at 3,6,12,24 PBH. Resuits The activity of myocardial NF-κB in bum groups was markedly increased at 1 PBH [ （20.27± 3.43） × 10^4 A], which was obviously higher than that in control group[ （2.18 ＋ 0.38）× 10^4 A, P 〈 0.01 ], and was still higher than that in control group at 24 PBH （ P 〈0. 01 ）. The expressions of myocardial IL-8 and ICAM-1 mRNA and avtivity of myocardial myeloperoxidse were obviously higher than that in control group at 3,6,12,24 PBH respectively （ P 〈 0.01 ）, LVSP and ＋ dp/dtmax significantly were lower, but LVEDP was higher than that in control group during 3 - 24 PBH （ P 〈 0.01 ）. Compared with that in burn group, these indices were ameliorated in PIYI~ group. Conclusions Severe burn might activate myocardial NF-κB, which ultimately lead to the production of cytokines, PMN aggr

关 键 词：烧伤 心肌 核因子-ΚB 

分 类 号：R285.5[医药卫生—中药学]