Tb掺杂的纳米羟基磷灰石生物荧光探针的合成与表征  

Synthesis and Characterization of Terbium-doped Nano Hydroxyapatite Biological Florescent Probe

作　　者：李玲[1] 刘玉侃[2] 陶锦晖[1] 张铭[2] 潘海华[1] 唐睿康[1] 

机构地区：[1]浙江大学化学系浙江大学生物物质与信息调控研究中心 [2]浙江大学生命科学院,杭州310058

出　　处：《无机化学学报》2008年第9期1369-1373,共5页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金资助项目(No20571064、20701032)

摘　　要：本文在室温下用Tb(Ⅲ)对纳米羟基磷灰石(HAP)进行表面修饰制成了新型无机生物荧光探针。在十六烷基三甲基溴化铵(CTAB)参与调控下,控制适当的反应条件,可生成20nm左右的HAP颗粒。经表面修饰后所得的Tb-HAP颗粒的尺寸范围仍在20±5nm之间,但具有发光特性,其最大发射峰值在544nm,且能在可见光波段(488nm)下进行激发。生物TEM结果表明,这种20nm左右大小的Tb-HAP可以很容易地进入兔脊髓间充质干细胞。因此,这一具有荧光特性的Tb-HAP有望成为具有良好生物兼容性的并可在细胞内稳定存在的生物无机纳米探针。An inorganic biological florescent probe, Tb（Ⅲ）-doped HAP nanoparticles was obtained by partially replacing calcium ions with terbium ions on the surface of 20 nm HAP at room temperature. Cetyhrimethylammonium bromide （CTAB） was used in the precipitation of hydroxyapatite to regulate the particle size and the synthesized HAP had a relatively uniform size of 20 nm. Calcium ions on the surface of HAP particle could be partially replaced by Tb（Ⅲ） to result in the Tb-HAP nanoparticles. The sizes of the Tb-HAP particles are 20±5 nm. After the surface treatment, the nanoparticles become luminescent and their maximum emission intensity is observed at 544 nm, which could be also excited at a visible light region of 488 nm. The result of the biological transmission electron microscopy confirms that these Tb-HAP nanoparticles could be internalized by the rabbit mesenchymal stem cells readily. Therefore, it suggested that the Tb-HAP nanoparticles could be used as a suitable nano inorganic florescent probe with excellent biocompatibility and stability in cells.

关 键 词：羟基磷灰石 铽 荧光 生物探针 纳米颗粒 

分 类 号：O614.231[理学—无机化学] O614.341[理学—化学]