黄瓜ω-3脂肪酸去饱和酶基因cDNA的克隆及表达  

Molecular Cloning and Expression Analysis of Fatty Acid Desaturase Gene in Cucumber

作　　者：刘春香[1] 何启伟[2] 赵光强[1] 阚世红[1] 张春兰[1] 

机构地区：[1]潍坊学院生物工程学院,山东潍坊261061 [2]山东省农业科学院蔬菜研究所,济南250100

出　　处：《园艺学报》2008年第9期1357-1362,共6页Acta Horticulturae Sinica

基　　金：潍坊学院院级项目(2004k16)

摘　　要：利用cDNA末端快速扩增技术,从黄瓜叶片组织中获得脂肪酸去饱和酶基因(Cs-FAD),其序列长度为1807bp,编码445个氨基酸,推导的氨基酸序列与已经报道的ω-3脂肪酸去饱和酶基因序列同源性较高,与桃果实的同源性最高(77%)。利用RT-PCR半定量分析显示,该基因在绿色组织如茎、叶、卷须里表达量较高,在根、花、果实及种子里也有少量表达。The fatty acid desaturase gene （Cs-FAD）, which full-length is 1 807 bp and encode 445 amino acids, was cloned from cucumber leaves by technique of rapid PCR amplification cDNA-ends. High similarity was observed between the sequence of Cs-FAD and FAD of peach （identity, 77% ）. The result of RT-PCR simi-quantitative analysis showed that Cs-FAD expresses more highly in green tissues, such as leaves, stems, hooks and so on. And it could also be found expression in roots, flowers, fruits and seeds, in a low level.

关 键 词：黄瓜 基因 克隆 表达 风味 抗性 

分 类 号：S642.2[农业科学—蔬菜学]