白簕愈伤组织的诱导及褐化的防治  

Callus Inducement of Acanthopanax trifoliate(L).Merr and Anti-Browning

作　　者：王箫[1] 黎云祥[1] 邹利娟[1] 余啊梅[1] 

机构地区：[1]西华师范大学生命科学学院,四川南充637002

出　　处：《西华师范大学学报（自然科学版）》2008年第3期263-268,共6页Journal of China West Normal University(Natural Sciences)

基　　金：四川省杰出青年学科带头人培养计划项目(04ZQ026-047);四川省科技厅应用基础项目(03JY029-021-22);四川省重点学科项目资助(SZD0420)

摘　　要：研究了不同培养条件、激素和外植体对白簕愈伤组织诱导的影响,并探讨其愈伤组织褐化的防治.结果表明:白簕愈伤组织诱导的适宜外植体为叶片,叶片愈伤组织诱导的最佳培养基为MS+2,4-D 1.0 mg/L+6-BA1.0 mg/L,诱导率高达92.7%.在继代培养中,采用固体培养对防止愈伤组织的褐化效果最佳,在培养基中分别添加l g/L的活性炭1g/LPVP,也可以有效防止褐化.A study on callus inducement from Acanthopanax trifoliate （L）. Merr explants has been conducted with different mediums and hormones. The optimal explants for A. trifoliate tissue culture are the leaf and the culture mediums are MS ＋ 2,4 - D 1.0 mg/L ＋ 6 - BA 1.0 mg/L which inducement rate is 92.7%. During subeu!ture, the solid medium has been proved to be the best mediums for anti-browning of callus. Adding 1 g/L activated carbon and 1 g/L PVP, that effect has also been found .

关 键 词：白簕 愈伤组织 外植体 褐化 

分 类 号：Q944.6[生物学—植物学]