对虾Taura综合征病毒RT-PCR检测方法的改进及应用  

Improvement and application of reverse transcription-polymerase chain reaction (RT-PCR) for the detection of taura syndrome virus in Penaeus vannamei

作　　者：黎小正[1] 韦信贤[1] 吴祥庆[1] 黄国秋[1] 黄玉柳[1] 

机构地区：[1]广西渔业病害防治环境监测和质量检验中心,广西南宁530021

出　　处：《上海水产大学学报》2008年第5期525-529,共5页Journal of Shanghai Fisheries University

摘　　要：Taura综合征病毒(TSV)是世界动物卫生组织(OIE)目录规定的水生动物二类疫病病原体。本研究在RT-PCR方法检测TSV行业标准的基础上,优化建立了能更准确检测TSV的套式RT-PCR。敏感性试验结果表明,所建立的套式RT-PCR的灵敏度大约为常规RT-PCR的103倍,最低可检测到10fg的TSVRNA。分别应用两种RT-PCR方法对180份来自广西沿海不同对虾养殖场的对虾临床样品进行检测,其中套式RT-PCR共有52份检出TSV,而常规RT-PCR仅有23份检出TSV。表明所建立的套式RT-PCR提高了TSV的阳性检出率,较常规RT-PCR有更大的应用价值和意义。Taura syndrome virus （TSV） was defined as List B Diseases pathogen of aquatic animals by Office International des Epizooties （OIE）. A nested RT-PCR was developed and optimized for the detection of TSV, founding on the industry standard for RT-PCR of TSV. By sensitivity analysis, the nested RT-PCR developed was 3-log more sensitive than that of conventional RT-PCR for the detection of TSV, and a minimum of 10fg RNA was detected. Using the two RT-PCR techniques, TSV was detected in 52 of 180 clinical cultivated penaeid shrimps in Guangxi by nested RT-PCR, while 23 of 180 by conventional RT-PCR. Results demonstrated that the nested RT-PCR developed can boost positive detection rate of TSV, and be a more effective diagnostic tool in detecting TSV.

关 键 词：TAURA综合征病毒 套式RT-PCR 灵敏度 阳性检出率 

分 类 号：S917[农业科学—水产科学]