应用噬菌体生物扩增技术检测牛结核分枝杆菌的方法研究  

Application of Bacteriophage Biological Amplification Technique for the Rapid Detection of Mycobacterium bovis

作　　者：刘子芝[1] 赵勇[1] 刘纪艳[1] 杨建华[1] 尹望中 王颖[1] 赵晶[1] 周永燚 

机构地区：[1]天津市动物检疫站,300211

出　　处：《中国动物检疫》2008年第10期20-21,共2页China Animal Health Inspection

基　　金：天津市科学技术委员会资助(06YFJMJC11900)

摘　　要：建立噬菌体生物扩增技术检测牛结核分枝杆菌试验方法,探讨最佳检测条件。比较各种检测方法对测定结果的影响。结果:噬菌体工作浓度为1×108PFU/ml、37℃感染2h,杀毒剂浓度在100mmol/L、室温作用10min为最佳检测条件。指示细胞浓度在1×106条/ml时,形成噬菌斑清晰,便于计数。结论:在选择最佳测定条件下,噬菌体生物扩增法检测牛结核分枝杆菌,快速、简便、灵敏。Bacteriophage biological amplification technique was established for the detection ot Mycobacterium bovis, the optimal test conditions were discussed. Result：the optimal test conditions were the working backerophage concentration [x]10^8PFU/mL, infection at 37℃ for 2 hours and germicide （ferrous ammouium sulfate） concentration 100mmol/L at room temperature for 10 minutes. When the index cell concentration was 1 ×10^6/mL, the phage plagues were clear and easy to count. Conclusion： the bacteriophage bio-amplification method was rapid, simple and sensitive for the detection of Mycobacterium boris under the selected optimal conditions.

关 键 词：噬菌体 牛结核分枝杆菌 检测方法 

分 类 号：S852.618[农业科学—基础兽医学]