高效液相色谱-质谱联用法测定血浆富马酸卢帕他定浓度  

Determination of Plasma Concentration of Rupatadine Fumarate by HPLC-MS/MS

作　　者：严慧娟[1] 李茜[1] 乔建[1] 邓俊刚[1] 黄厚武[1] 黎维勇[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院药剂科,武汉430022

出　　处：《医药导报》2008年第10期1174-1176,共3页Herald of Medicine

摘　　要：目的建立高效液相色谱一质谱联用（HPLC-MS／MS）法测定人血浆富马酸卢帕他定浓度。方法以氯雷他定为内标，待测血浆样品经氢氧化钠碱化，加醋酸乙酯-二氟甲烷（4：1）萃取后，用液-质联用方法进行检测。结果血浆富马酸卢帕他定的浓度线性范围为0．05—13．00μg·L^-1，最低定量浓度为0．05μg·L^-1；提取回收率为65．06％-73．25％。结论该法分离效果良好，灵敏度高，回收率高，可用于人体血药浓度测定及药动学研究。Objective An HPLC-MS/MS method was established for the determination of rupatadine fumarate in plasma. Methods The drug and internal standard （loratadine）in plasma were alkalified with sodium hydroxide, extracted with acetic ether-dichloromethane （4 ： 1 ） and detected by HPLC-MS/MS. Results A good linearity of rupatadine fumarate in plasma was obtained from 0.05 to 13 μg · L^-1 , the minimum level of which was 0.05 μg · L^-1 and the recovery rate was from 65.06% to 73.25%. Conclusion The method is sensitive, accurate and reliable, and could be used for determing drug concentration and pharmacokinetic of rupatadine fumarate.

关 键 词：卢帕他定 高效液相色谱-质谱联用 血药浓度 

分 类 号：R976[医药卫生—药品] R927.2[医药卫生—药学]