二噁英对大鼠骨骼发育的抗雌激素作用  

Anti-estrogenic effect of dioxin on rat skeleton development

作　　者：郭磊[1] 赵玉岩[2] 张世亮[1] 刘魁[1] 高晓宇[1] 

机构地区：[1]中国医科大学附属第一医院骨科,沈阳110001 [2]中国医科大学附属第一医院内分泌科

出　　处：《卫生研究》2008年第5期563-565,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.40500414);辽宁省教育厅高等学校科学研究项目(No.05L508)

摘　　要：目的研究环境类致畸因子的拮抗雌激素的致畸作用。方法应用不同剂量二噁英(TCDD)构建先天SD大鼠骨骼发育畸形动物模型,茜素红染色法制作并观察透明骨骼标本,采用光镜观察胎鼠趾骨骨化中心的软骨细胞病理学变化,放射免疫法测定母鼠血清雌二醇的变化规律。结果TCDD在5～15μg/kg浓度下诱导了大鼠骨骼发育畸形,并存在剂量依赖性生物学效应。光镜下在畸形胎鼠的趾骨骨化中心可见软骨发生带缩小,软骨细胞变性,肥大软骨细胞数量明显减少;TCDD实验组的母鼠血清雌二醇含量明显高于正常对照组(P<0.05)。结论TCDD可能通过降低雌激素生物活性和干扰软骨细胞的代谢,引起大鼠肢端骨化中心的结构紊乱而发挥骨骼致畸效应。Objective To investigate the teratogenic and anti-estroglnic effect of enviroment teratogen on rat skeleton development.Methods The fetal rat models with congenital skeleton malformation were constructed with 2,3,7,8-tetrachlorodibenzo-p-dioxin（TCDD） on pregnant day 10.The transparent skeleton of fetal rat was made with alizarin bordeaux.The cell pathology of chondrocyte in fetal rat center of ossification was observed by light microscope.The estradiol in rat serum was measured by radioimmunity method.Results Treated with 5-15μg/kg TCDD,the single or multiple rat fetal skeletal development malformation had been induced.The teratogenic effect of TCDD on the osteogenesis was in dose-dependent manner.There were decreased center of ossification and cell degeneration of chondrocyte in fetal extremity limbs.The estradiol increased significantly in TCDD-treated rat,compared with normal rat（P〈0.05）.Conclusion TCDD can induce congenital fetal skeletal malformation under interfering the biological activity of estrogen and the function of chondrocyte and the primary center of ossification.

关 键 词：二噁英 雌激素 软骨细胞 畸形 骨骼发育 

分 类 号：R322.71[医药卫生—人体解剖和组织胚胎学] Q579.13[医药卫生—基础医学]