肥胖儿童血清中三种PAEs的检测和分析  

Determine and parallel analysis of three kinds of PAEs in serum for obese children

作　　者：李平[1] 代兴碧[1] 但洪[2] 黄晓红 蒲朝文 

机构地区：[1]重庆医科大学公共卫生学院基础医学研究所,重庆400016 [2]四川大英县人民医院 [3]四川平武县疾病预防控制中心 [4]重庆涪陵区疾病预防控制中心

出　　处：《卫生研究》2008年第5期581-583,共3页Journal of Hygiene Research

基　　金：重庆医科大学校级启动基金

摘　　要：目的了解10～12岁肥胖儿童血清中3种邻苯二甲酸酯类(PAEs)——邻苯二甲酸二乙酯(DEP)、邻苯二甲酸二丁酯(DBP)和邻苯二甲酸二异辛酯(DEHP)的含量。评估三种PAEs对肥胖儿童可能产生的危害。方法运用反相高效液相色谱分析法(HPLC)测定10～12岁肥胖儿童36名和正常体重儿童36名血清中三种PAEs含量,对两人群三种PAEs测定值(mg/L)作对比分析及性别差异分析。结果肥胖儿童血清中三种PAEs的中位数(mg/L)分别为0.0032(DEP)、0.1649(DBP)和0.1680(DEHP);正常体重儿童血清中三种PAEs的中位数(mg/L)分别为0.0026(DEP)、0.0359(DBP)和0.1063(DEHP);经Wilcoxon两样本检验,Z值分别为1.1013、5.5584和3.0128,P值分别为0.2708、<0.0001和0.0026,肥胖儿童与正常体重儿童血清中DBP和DEHP的含量差异有统计学意义,而DEP含量差异无统计学意义。两组儿童的含量构成比经χ2检验,χ2值分别为18.57、34.5956和10.9193,P值分别<0.0001、<0.0001和0.0043,肥胖儿童与正常体重儿童血清中DEP、DBP和DEHP的含量构成比均有统计学意义。结论肥胖儿童DBP、DEHP的含量平均水平显著高于正常体重儿童,且肥胖儿童在含量较高水平上的人数多于正常体重儿童。Objective To study the serum contents of the PAEs of obese children at the ages of 10 to12 years,in order to estimate the harm of PAEs on obese children.Methods The contents of three kinds PAEs（DEP,DBP and DEHP） in the serum for two groups of children,including 36 obese children and 36 normal weight children,were determined by the reversed phase high performance liquid chromatography（RP-HPLC） method And the average measure value of three kind PAEs between two groups of children were analysed.Results The median serum levels of PAEs were 0.0032（DEP）,0.1649（DBP） and 0.1680（DEHP） in obese children.And the serum levels of PAEs were 0.0026（DEP）,0.0359（DBP） and 0.1063（DEHP） in normal weight children.The differences of average measure value of DBP and DEHP in three kind PAEs between two groups of children were significant（P〈0.01）.The amounts of obese children in high level were more than those of normal weight children,and the constitution ratios in three kinds of PAEs of obese children were higher than those of normal weight children.The differences between two groups of children were significant（P〈0.01）.Conclusion The average levels of DBP and DEHP in serum of obese children were more than those of serum of normal weight children.The amounts of obese children were higher than those of normal weight children in high level content of three kinds of PAEs.

关 键 词：肥胖儿童 血清 邻苯二甲酸酯类 高效液相色谱 

分 类 号：R114[医药卫生—卫生毒理学] X503.1[医药卫生—公共卫生与预防医学]