HPV16／18阳性的宫颈尖锐湿疣及癌前病变组织中NF-κBp50和P16^INK4A蛋白表达分析  

EXPRESSION OF NF-ΚBP50 AND P16^(INK4A) IN CONDYLOMATA ACUMINATA AND CERVICAL INTRAEPITHELIAL NEOPLASIA WITH HPV16/18

作　　者：金泽龙[1] 谭超[2] 董丹丹[3] 李科[3] 李文竹[3] 

机构地区：[1]四川省雅安市职业技术学院附属医院皮肤科,雅安625000 [2]兰州大学第二医院皮肤科 [3]四川省人民医院病理科

出　　处：《现代预防医学》2008年第19期3853-3854,3857,共3页Modern Preventive Medicine

摘　　要：[目的]探讨NF-κBp50和P16INK4A在HPV16/18型宫颈尖锐湿疣(condylomata acuminate CA)和宫颈上皮内瘤变(cervical intraepithelinal neoplasin CIN)组织中的表达及意义。[方法]应用免疫组织化学LDP法对21例宫颈CA、34例宫颈CIN进行NF-κBp50和P16INK4A表达分析。[结果]NF-κBp50在CA组织中阳性表达于细胞浆,在CIN组织中阳性表达于细胞核,NF-κBp50在CA、CINⅠ、CINⅡ3组间阳性表达强度有统计学意义(P﹤0.05),P16INK4A在CA组织中阳性表达于细胞核,在CIN阳性表达于细胞核与细胞浆,P16INK4A在CA、CINⅠ、CINⅡ3组间阳性表达强度有统计学意义(P﹤0.05)。[结论]HPV16/18阳性CA向CIN转化可能与P16INK4A和NF-κBp50蛋白过渡表达有关;其联合检测可作为辅助诊断CIN的标志物。[ Objective] To evaluate the significance of NF-κBp50 and P16^INK4A protein expression in the condyloma acuminatum （CA） and cervical intraepithelial neoplasia （CIN）. [Methods] 21 cases ca and 34 cases CIN tissue were detected the expression of P16P16^INK4A and NF-κBp50 by immunohistochemical analysis （LDP）. [ Results] By using immunohistochemical analysis, NF-κBp50 protein expression in CA was mainly localized in cytosol, NF-κBp50 protein expression in CIN was mainly localized in nucleus. NF-κBp50 protein expression in CA, CIN Ⅰ and CIN Ⅱ was different （P〈 0.05）. P16^ INK4A protein expression in CA was mainly localzed in nucleus. P16^INK4A protein expression in CIN was mainly localized in nucleus and cytosol. P16^INK4A protein expression in CA, CIN Ⅰ and CIN Ⅱwas different （P 〈 0.05）. [Conclusion] The conversion of HPV16/18 positive CA to CIN is correlated with P16^INK4A and NF-kappa Bp50 protein high expression. Their combined detection can be used as the auxiliary diagnostic markers of CIN Key words：

关 键 词：尖锐湿疣 宫颈上皮内瘤变 HPV16/18 P16^INK4A NF-ΚBP50 

分 类 号：R759[医药卫生—皮肤病学与性病学] R737.33[医药卫生—临床医学]