人类β1,3半乳糖基转移酶7对人4IgB7-H3的修饰作用初探  

Primary study of UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase polypeptide 7′s modification to human 4IgB7-H3

作　　者：唐春花[1] 马震宇[1] 岳爱环[1] 行书丽[1] 蒋俊华[1] 张昊[2] 曹静媛[2] 周迎会[1] 吴士良[1] 

机构地区：[1]苏州大学基础医学与生物科学学院生物化学与分子生物学教研室,生化工程研究所,江苏苏州215123 [2]苏州大学医学部,江苏苏州215123

出　　处：《江苏大学学报（医学版）》2008年第5期378-382,I0001,共6页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(30771958;30670462)

摘　　要：目的:探讨人4IgB7-H3基因转染人胃癌细胞株SGC7901后人类β1,3半乳糖基转移酶7(β1,3-galacto-syltransferase 7,β3GalT7)的表达变化,以期发现4IgB7-H3与β3GalT7的相互关系。方法:脂质体介导重组逆转录病毒载体pEGZ-Term/4IgB7-H3转染SGC7901细胞,筛选获得稳定表达4IgB7-H3的亚克隆细胞株,通过RT-PCR和蛋白质印迹方法检测β3GalT7的表达。结果:成功获得稳定表达4IgB7-H3的亚克隆细胞株SGC7901/4IgB7-H3;β3GalT7随4IgB7-H3的表达上调而上调。结论:4IgB7-H3蛋白的修饰成熟可能需要β3GalT7的作用。Objective： We researched the change of UDP-Gal：betaGlcNAc beta 1,3-galactosyltransferase polypeptide 7 expression in SGC7901/4IgB7-H3 cell to find the relation between 4IgB7-H3 and 133GalT7. Methods： Transfered pEGZ-Term/4IgB7-H3 into SGC7901 cell, then detected the expression of UDP-Gal：betaGlcNAc beta i ,3-galactosyltransferase polypeptide 7 with RT-PCR and Western-blotting. Results： SGC7901/4IgB7-H3 cell line were successfully obtained. The expression of β3GalT7 was upregulated follow the up-regulation of 4IgB7-H3 on mRNA and protein level. Conclusion： UDP-Gal：beta- GlcNAc beta 1,3-galactosyhransferase polypeptide 7 may modify 4IgB7-H3 protein.

关 键 词：4IgB7-H3 β1 3半乳糖基转移酶7 SGC7901 转染 

分 类 号：Q5[生物学—生物化学]