驼背鲈线粒体细胞色素b基因的序列分析  

Sequence analysis of mitochondrial cytochrome b gene of high-finned grouper (Cromileptes altivelis)

作　　者：区又君[1] 李加儿[1] 吴勇[1,2] 刘楚吾[2] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]广东海洋大学水产学院,广东湛江524088

出　　处：《南方水产》2008年第5期51-54,共4页South China Fisheries Science

基　　金：国家高技术研究发展计划(863计划)项目(2006AA10A414);广东省科技计划项目(C20817);广东省重大科技兴海项目(A200501A04);中国水产科学研究院基金项目(2002-3-1)

摘　　要：采用PCR-DNA测序技术,对驼背鲈线粒体DNA细胞色素b(Cytb)基因全序列进行分析。获得长度为1141bp的Cytb基因片段,序列碱基组成平均是T为29.1%,C为32.3%,A为23.2%,G为14.0%,A+T的碱基百分比总体上略大于C+G,平均值分别是52.3%和46.3%。与其它鱼类相同基因片段碱基序列含量相似。在由核苷酸推导的氨基酸中,亮氨酸(Leu)所占的比例最高,为16.03%。Complete sequence of mitochondrial DNA cytochrome b （ Cyt b） gene in high-finned grouper （ Cromileptes altivelis） was analyzed with PCR-sequencing. The 1 141 bp of Cyt b gene in C. altivelis was obtained. Base composition of Cyt b gene in C. altivelis were 29. 1% , 32. 3%, 23.2% , 14. 0% for T, C, A and G, respectively, with 52. 3% and 46. 3% for A ＋ T and C ＋ G, respectively, which were similar to that in other fishes. The corresponding amino acid sequence was deduced and the percentage of Leu was 16.03% , much higher than any other amino acid.

关 键 词：驼背鲈 细胞色素b(Cyt b) 序列 

分 类 号：Q244[生物学—细胞生物学]