熟地黄对D-半乳糖衰老模型大鼠脑细胞周期蛋白表达的影响  

Effects of Prepared Radix Rehmanniae on Brain Cell Cycle and Related Gene Expression

作　　者：安红梅[1] 史云峰[1] 胡兵[1] 史秀峰[1] 张占鹏[1] 许丽雯[1] 顾明昌[1] 

机构地区：[1]上海中医药大学附属龙华医院,上海2001032

出　　处：《中国药师》2008年第10期1145-1147,共3页China Pharmacist

基　　金：上海市教委青年基金项目(基金编号:04CC19)

摘　　要：目的:从脑细胞周期的角度揭示熟地黄防治脑衰老的作用机制。方法:36只SD大鼠随机分为正常组、模型组、天保宁组和熟地黄组,采用D-半乳糖造模同时服用相应药物45d。在检测大鼠行为学、脑组织生化指标、细胞衰老特异代谢产物SA-β-gal阳性表达细胞数的基础上,采用流式细胞术检测细胞周期、免疫组化法检测海马CA1区P53,P21,P16的表达。结果:熟地黄能一定程度促进细胞周期从G0/G1期向S期进展,抑制海马CA1区CDK抑制基因蛋白P53,P21,P16的表达。结论:熟地黄能调控细胞周期相关基因P53,P21,P16表达。Objective： To study the mechanism of prepared Radix Rehmanniae （ShuDi） on brain ageing based on cell cycles. Method： Thirty-six Sprague-Dawley rats were divided into 4 groups randomly, that were normal, model, Tianbaoning and prepared Radix Rehmanniae （ShuDi） group. Besides prophase study on rats inteUigence,biochemical changes of brain tissue and special products SA-β-gal of brain cells,cell cycles were further determined by flow cytometry and P53, P21 and P16 express in CA1 hippocampus zone were also observed by immunohistochemistry. Result： Prepared Radix Rehmanniae （ShuDi） could prompt the progression of cell cycles in some extent,decrease the expression of P53, P21 and P16 in CA1 hippocampus zone were decreased. Conclusion： Prepared Radix Rehmanniae （ShuDi） could regulate cell cycle related gene P53, P21 and P16 expression in CA1 hippoeampus zone.

关 键 词：熟地黄 脑衰老 海马CAI区 细胞周期 P53 P21 P16 

分 类 号：R285.5[医药卫生—中药学]