生姜组织培养中脱菌试验研究  

Study on Sterilization Trial of Tissue Culture in Ginger

作　　者：薛寒青[1] 王舰[1] 

机构地区：[1]青海省农林科学院生物所,青海西宁810016

出　　处：《长江蔬菜》2008年第09X期60-62,共3页Journal of Changjiang Vegetables

摘　　要：选用姜块进行3步复合消毒法脱菌试验,试验结果表明,试验最佳处理组合为0min5%次氯酸钠(NaClO)、0.5min70%乙醇(C2H5OH)和15min0.1%升汞(HgCl2)。Explant of ginger has high polluting rate in tissue culture, which is the keystone for the tissue culture. Multiple factor experiments for stem apex of ginger were made in order to reduce the polluting rate, to increase induction rate of adventitious bud and survival ratio of stem apex of ginger. Three steps of complex sterilization in the experiment were used, the results showed that the combination of 0 min 5% NaClO, 0.5 rain 70% C2H5OH and 15 min 0.1% HgCl2 was the best.

关 键 词：生姜 组织培养 脱菌 

分 类 号：S632.5[农业科学—蔬菜学] Q943.1[农业科学—园艺学]