乙肝病毒X蛋白诱导细胞凋亡的研究  

Study of hepatitis B virus X protein inducing cell apoptosis

作　　者：刘昌勇[1] 李正[1] 李小玉[2] 刘豫蓉[2] 胡火珍[1] 

机构地区：[1]四川大学生命科学学院医学细胞生物学教研室 [2]口腔疾病研究国家重点实验室,四川大学,四川成都610041

出　　处：《国际口腔医学杂志》2008年第5期491-493,605,共4页International Journal of Stomatology

摘　　要：目的探讨乙肝病毒X蛋白在诱导细胞凋亡中的作用。方法用基因重组的方法构建乙肝病毒X(HBX)真核表达载体,脂质体转染体外培养的正常小鼠胎肝细胞(FL)、人肝癌细胞(SMMC-7721)和人宫颈癌细胞(Hela);用免疫组化染色法检查HBX基因的表达;用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法及流式细胞术检测细胞凋亡。结果成功构建X基因真核表达载体,经免疫组化检测,重组载体pcDNA3-X均可在以上3种不同类型细胞中表达,并能诱导Hela细胞凋亡。结论HBX能诱导细胞凋亡,获得稳定表达HBX蛋白的Hela细胞株,经传代20代仍然稳定表达HBX蛋白。Objective To investigate the role of hepatitis B virus X （HBX） protein in cell apoptosis. Methods Eukaryotie expression vector of HBX gene was constructed. Recombination vector mentioned above was transfected by Lipofeetamine to Hela cell, Hepatocarcinoma cell （SMMC-7721） and normal mouse fetal liver cell （FL）. X protein （pX） expressed in these three cell type was detected by immunohistochemieal method. The apoptosis in Hela-X cells was analyzed by flow cytometry and the terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate fluorescence nick end labeling（TUNEL） method. Results In three cell type, pX expressed. A stable pX-expression cell line was selected by G418. HBX protein could express in Hela ceils in 20 passages. Conclusion HBX protein could induce apoptosis in vitro.

关 键 词：乙肝病毒X蛋白 聚合酶链反应 真核表达载体 细胞凋亡 

分 类 号：R373[医药卫生—病原生物学]