注射携人IGF-1基因的成肌细胞对小鼠损伤骨骼肌内源性mIGF-1表达的影响  

The Effect of Myoblast Carrying Human IGF-1 Injection on the Endogenous mIGF-1 mRNA Expression in Mice Skeletal Muscle following Injury

作　　者：李宏云[1] 陈世益[1] 陈疾忤[1] 卫宏图[1] 张鹏[1] 李云霞[1] 

机构地区：[1]复旦大学运动医学中心复旦大学附属华山医院运动医学科,上海200040

出　　处：《中国运动医学杂志》2008年第5期584-587,共4页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金(30170446);上海市医学"百人计划"(97BR017)资助

摘　　要：目的：研究携人胰岛素样生长因子-1（human insulin—like growth factor-1，hIGF-1）基因的成肌细胞移植损伤小鼠体内后，内源性mIGF-1mRNA及mIGF-1因子的表达情况。方法：雄性C3H小鼠（20～30g，7～11周）共76只随机分为3组：A组（转基因成肌细胞移植组）、B组（空白成肌细胞移植组）、C组（生理盐水对照组），每组24只。另4只鼠作正常对照。A、B、C各组小鼠于右下肢腓肠肌内侧面中段实施钝性打击，打击伤后第3天分别于致伤部位注入1×10^6个转基因成肌细胞、等量的空白成肌细胞或100μl生理盐水；打击伤后第2、5、10、15、20、30天，于各组中随机抽取4只小鼠处死，取右侧腓肠肌中段进行检测，以real time RT—PCR和免疫组化染色检测mIGF-1的表达。结果：（1）各组小鼠体内均有mIGF-1mRNA的表达、mIGF-1免疫组化染色阳性；（2）A组小鼠mIGF-1mRNA的表达和mIGF-1因子的分泌明显高于B、C组。结论：携hIGF-1基因的成肌细胞移植入钝挫伤的小鼠体内后，可促进mIGF-1 mRNA的表达和mIGF-1因子的分泌。Objective To observe the effect of the injection of myoblast carrying human insulin-like growth factor-1 （hlGF-1） on the expression of endogenous IGF-1 mRNA and IGF-1 level in mice skeletal muscle following injury. Methods Seventy two male C3 H mice （20 -30g, 7 - 11 w） were randomly divided into three groups （24 mice in each group） with four mice normal controls. Applied a falling hit from certain height at the medial calf of right lower limbs in three groups, the injured skeletal muscle model was successfully simulated. Three days following injury, the mice in group A and B were injected with 1 × 10^6 myoblasts either carried with or without hlGF-1 gene respectively and the mice in group C were injected with 100/21 saline at the injured muscle. Three mice in each group were sacrificed randomly at day 2, 5, 10, 15, 20, 30 after contusion. The expression level of mIGF-1 was assessed by immunohistochemical staining and real time PCR. Results mlGF-1 mRNA expression and mlGF-1 factor secretion were observed in all three groups; the amount of mlGF-1 mRNA expression and mlGF-1 secretion in group A were significant higher than that in group B and C. Conclusion Myoblast carrying hlGF-1 transplantation could promote endogenous IGF-1 secretion in injured skeletal muscle.

关 键 词：胰岛素样生长因子-1 成肌细胞 骨骼肌 损伤 修复 

分 类 号：R873[医药卫生—运动医学]