Clinical, virologic and phylogenetic features of hepatitis B infection in Iranian patients  

作　　者：Golnaz Bahramali Majid Sadeghizadeh Samad Amini-Bavil-Olyaee Seyed-Moayed Alavian Abbas Behzad-Behbahani Ahmad Adeli Mohammad-Reza Aghasadeghi Safieh Amini Fereidoun Mahboudi 

机构地区：[1]Department of Genetics,Faculty of Basic Sciences,Tarbiat Modares University,Tehran 14115-175,Iran [2]Biotechnology Department,Pasteur Institute of Iran,Tehran 13164,IranMohammad-Reza Aghasadeghi,Safieh Amini,Hepatitis and HIV Department,Pasteur Institute of Iran,Tehran 13164,Iran [3]Baqiyatallah Research Center for Gastroenterology and Liver Diseases,Tehran Hepatitis Center,Tehran 14155-3651,Iran [4]Medical Technology Research Center,School of Paramedical Sciences,Shiraz University of Medical Sciences,Shiraz 7143914693,Iran [5]Biotechnology Department,Pasteur Institute of Iran,Tehran 13164,Iran [6]Hepatitis and HIV Department,Pasteur Institute of Iran,Tehran 13164,Iran

出　　处：《World Journal of Gastroenterology》2008年第35期5448-5453,共6页世界胃肠病学杂志（英文版）

基　　金：A grant from the Nanotechnology committee of the Ministry of Science, Research and Technology, Iran, No. 31.1895 on 05.03.2004 to Majid Sadeghizadeh

摘　　要：AIM： To characterize the clinical, serologic and virologic features of hepatitis B virus （HBV） infection in Iranian patients with different stages of liver disease.METHODS： Sixty two patients comprising of 12 inactive carriers, 30 chronic hepatitis patients, 13 patients with liver cirrhosis and 7 patients with hepatocellular carcinoma （HCC） were enrolled in the study. The HBV S, C and basal core promoter （BCP） regions were amplified and sequenced, and the clinical, serologic, phylogenetic and virologic characteristics were investigated.RESULTS： The study group consisted of 16 HBeAgpositive and 46 HBeAg-negative patients. Anti-HBepositive patients were older and had higher levels of ALT, ASL and bilirubin compared to HBeAg-positive patients. Phylogenetic analysis revealed that all patients were infected with genotype D （mostly ayw2）. The G1896A precore （PC） mutant was detected in 58.1% patients. HBeAg-negative patients showed a higher rate of PC mutant compared to HBeAg-positive patients （2,2 = 9.682, P = 0.003）. The majority of patients with HCC were HBeAg-negative and were infected with PC mutant variants. There was no significant difference in the occurrence of BCP mutation between the two groups, while the rate of BCP plus PC mutants was higher in HBeAg-negative patients （2,2 = 4.308, P = 0.04）. In the HBV S region, the genetic variability was low, and the marked substitution was P120T/S, with a rate of 9.7% （n = 6）.CONCLUSION： In conclusion, HBV/D is the predominant genotype in Iran, and the nucleotide variability in the BCP and PC regions may play a role in HBV disease outcome in HBeAg-negative patients.AIM: To characterize the clinical, serologic and virologic features of hepatitis B virus (HBV) infection in Iranian patients with different stages of liver disease. METHODS: Sixty two patients comprising of 12 inactive carriers, 30 chronic hepatitis patients, 13 patients with liver cirrhosis and 7 patients with hepatocellular carcinoma (HCC) were enrolled in the study. The HBV S, C and basal core promoter (BCP) regions were amplified and sequenced, and the clinical, serologic, phylogenetic and virologic characteristics were investigated. RESULTS: The study group consisted of 16 HBeAg- positive and 46 HBeAg-negative patients. Anti-HBe- positive patients were older and had higher levels of ALT, ASL and bilirubin compared to HBeAg-positivepatients. Phylogenetic analysis revealed that all patients were infected with genotype D (mostly ayw2). The G1896A precore (PC) mutant was detected in 58.1% patients. HBeAg-negative patients showed a higher rate of PC mutant compared to HBeAg-positive patients (χ2 = 9.682, P = 0.003). The majority of patients with HCC were HBeAg-negative and were infected with PC mutant variants. There was no significant difference in the occurrence of BCP mutation between the two groups, while the rate of BCP plus PC mutants was higher in HBeAg-negative patients (χ2 = 4.308, P = 0.04). In the HBV S region, the genetic variability was low, and the marked substitution was P120T/S, with a rate of 9.7% (n = 6). CONCLUSION: In conclusion, HBV/D is the predominant genotype in Iran, and the nucleotide variability in the BCP and PC regions may play a role in HBV disease outcome in HBeAg-negative patients.

关 键 词：Hepatitis B virus Clinical and virologic features Genetic variability Phylogenetic analysis 

分 类 号：R512.62[医药卫生—内科学]