枸杞多糖对糖尿病大鼠肾脏PKC-βⅡ及IV型胶原表达的影响  

Effect of lycium barbarum polysaccharides on protein kinase C-βⅡ and collage IV of kidney in type 2 diabetic rats

作　　者：刘萍[1] 何兰杰[1] 

机构地区：[1]宁夏医学院附属医院内分泌科,宁夏银川750004

出　　处：《中国医院药学杂志》2008年第18期1544-1547,共4页Chinese Journal of Hospital Pharmacy

摘　　要：目的:研究枸杞多糖对2型糖尿病大鼠肾脏PKC-βⅡ及IV型胶原的影响。方法:制备2型糖尿病大鼠模型,将大鼠分为正常对照组,单纯糖尿病组,枸杞多糖干预组,检测糖尿病成型12周时各组大鼠肾脏肥大指数、尿微量白蛋白、肾功能、肾脏形态学指标及PKC-βⅡ、IV型胶原的表达。结果:与糖尿病组相比,枸杞多糖干预组肾肥大指数、尿微量白蛋白、肾功能、肾脏形态学指标均有改善,且PKC-βⅡ、IV型胶原的表达明显减低。PKC-βⅡ、IV型胶原与肾功能、肾脏形态学指标存在正相关。结论:枸杞多糖可以通过抑制PKC-βⅡ、IV型胶原的表达起到肾脏保护作用。OBJECTIVE To study the effect of LBP on protein kinase C-βⅡ and collage IV of kidney in type 2 diabetic rats. METHODS Type 2 diabetic rats model was established. The rats were derided into 3 groups： normal group, diabetic group without intervention, diabetic group with intervention orally by lyeium barbarum polysaccharides （LBP） （250 mg.kg-1 .d-1）. Abserve the kidney weight/body weight, urine microalbumin serum creatinine, blood urine nitrogen, the indexes of morpholo- gy, the expression of protein kinase C-βⅡ and collage IV after 12 weeks. RESULTS In LBP intervention group, the kidney weight/body weight, urine microalbumin, serum creatinine, blood urine nitrogen, the indexes of morphology were improved in comparison with diabetic rats. And the expression of protein kinase C and collage IV were reduced in comparison with diabetic rats. the kidney weight/body weight, urine microalbumin, serum creatinine, blood urine nitrogen, the indexes of morphology positively correlated with protein kinase C and collage Ⅳ. CONCLUSION LBP can protect the kidney of diabetic rats through reducing the expression of protein kinase C and collage Ⅳ.

关 键 词：枸杞多糖 蛋白激酶C Ⅳ型胶原 

分 类 号：R587.1[医药卫生—内分泌]