舟山渔场及其相邻赤潮高发区麻痹性贝类毒素研究  

THE SHELLFISH-BEARING PSP TOXIN IN AREAS OF HIGH RED TIDE OCCURRENCE OF THE ZHOUSHAN FISHING GROUND

作　　者：胡颢琰 唐静亮 黄备 毛宏跃 王婕妤 魏娜 

机构地区：[1]浙江省舟山海洋生态环境监测站,舟山316000

出　　处：《海洋与湖沼》2008年第5期475-481,共7页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金重大研究计划"全球变化及其区域响应";190511005号;浙江省环境保护局科研资助项目;200514号;200607号。

摘　　要：采用小白鼠生物检测法和高效液相色谱荧光检测法,对采自舟山渔场及其相邻赤潮高发区贝类的麻痹性贝毒素进行了调查分析与研究。结果表明,浙江舟山海域有毒贝类检出率为7.0%。利用高效液相色谱荧光检测法对2006年5月份采集的六横的西格织纹螺、岱山的毛蚶组织提取液进行麻痹性贝毒素测定,结果显示,六横的西格织纹螺体内麻痹性贝毒素含量为0.178Mu/g,毒力值为0.0356μg STXeq/g,其毒素成分由GTX2、GTX3、STX、dcSTX、C2组成;岱山的毛蚶体内麻痹性贝毒素含量为3.494Mu/g,毒力值为0.6987μg STXeq/g,其毒素成分由STX、NEO、C1、C2组成。浙江中、南部海域有毒贝类检出率为2.2%,利用高效液相色谱荧光检测法对2006年10月份在南麂列岛采集的棒锥螺的组织提取液进行麻痹性贝毒素的测定,结果显示棒锥螺体内麻痹性贝毒素含量为0.705Mu/g,毒力值为0.1409μg STXeq/g,其毒素成分有GTX1、GTX2、GTX3、NEO、C1、C2组成。PSP （paralytic shellfish poisoning） toxin is a biological toxin generated in dinoflagellates-oflen the members of red tide, and is accumulated in marine shellfish body. 12 artificial and wild shellfish species were sampled in the Zhoushan Fishing Ground of the Zhoushan Archipelago, China, a major seafood production base with frequent red-tide outbreaks, and adjacent regions in May to October 2006, to determine PSP toxicity levels in shellfish by AOAC bioassay and HPLC-FD. The results show that 7.0% of the samples were PSP-contaminated in Zhoushan area and 2.2% in offshore middle and southern Zhejiang. The HPLC-FD profile show that, in Nassarius siquinjorensis the concentration of PSP toxin was 0.178Mu/g, including major compositions of GTX2, GTX3, STX, dcSTX, and C2; in Scapharca subcrenata it was 3.494Mu/g, containing STX, NEO, C1, and C2; in Turritella bacillum it was 0.705Mu/g, consisting of GXT1, GXT2, GXT3, NEO, C1, and C2.

关 键 词：麻痹性贝毒素 赤潮 小白鼠生物检测法 高效液相色谱荧光检测法 舟山渔场 

分 类 号：S944.4[农业科学—水产养殖] X132[农业科学—水产科学]