毛细管电泳非接触电导检测分离测定水杨酸及其羟基化产物  

Separation and Determination of Salicylic Acid and Its Hydroxylating Products by Capillary Electrophoresis with Non-contact Conductivity Detection

作　　者：赵芳[1] 王立新[2] 傅崇岗[1] 

机构地区：[1]聊城大学化学化工学院,聊城252050 [2]聊城大学传媒技术学院,聊城252059

出　　处：《理化检验（化学分册）》2008年第9期848-850,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金(No.20675036);山东省自然科学基金(Y2006B04);山东省"泰山学者"建设工程专项经费

摘　　要：利用自组装的毛细管电泳非接触电导检测装置建立了水杨酸(SA,羟基自由基捕获剂)及其羟基化产物2,3-二羟基苯甲酸(2,3-DHBA)和2,5-二羟基苯甲酸(2,5-DHBA)的分离测定方法。在最佳条件下10min内即可完成分离,其线性范围为0.03～15.0mg·L^(-1)L^(-1),检出限(S/N =3)为0.01mg·L^(-1),对0.7mg·L^(-1)混合溶液进行测定,SA、2,3-DHBA和2,5-DHBA峰面积的相对标准偏差(n=8)依次为2.9%,3.2%,3.6%。Salicylic acid （SA）, a common siezing agent for hydroxyl free radical （ · OH）, and its hydroxylating products with the · OH free radical, i. e. , 2, 3-dihydroxyhenzoic acid （2, 3-DHBA） and 2, 5- dihydroxyhenzoic acid （2,5-DHBA）, were separated and determined by capillary electrophoresis with non-contact conductivity detection. Under optimum conditions, the above mentioned compounds were separated and detected within 10 min. Linear relationship between response signal and concentration of either of the 3 compounds was kept in the range of 0. 03--15. 0 mg · L^-1 , with detection limits of 0. 01 mg · L^-1. Test for precision was made at the concentration level of 0. 7 mg· L^-1 of each of the 3 compounds with 8 determinations, value of RSD＇s for measurements of peak area were found to be 2. 9% （for SA）, 3. 2% （for 2,3-DHBA） and 3.6% （for 2,5-DHBA）.

关 键 词：毛细管电泳 非接触电导检测 水杨酸 羟自由基 羟基化产物 

分 类 号：O657[理学—分析化学]