高效的苜蓿组织培养再生体系的建立  

An effective method of embryo induction and regeneration in Medicago sativa L.cv.Baoding

作　　者：王涌鑫[1] 关宁[1] 李聪[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100094

出　　处：《东北师大学报（自然科学版）》2008年第3期112-117,共6页Journal of Northeast Normal University(Natural Science Edition)

基　　金：国家科技支撑计划项目(2006BAD01A19);新疆草地资源与生态重点实验室开放课题资助项目(XJDX0201-2005-03)

摘　　要：以保定苜蓿为实验材料,从外植体的基因型、培养基的选择,激素(KT)、水解酪蛋白的浓度等方面探讨了影响保定苜蓿组织培养的因素.结果表明:下胚轴比子叶作为外植体获得的效果更好;改良的SH培养基相对于UM培养基对愈伤组织的诱导更有效;在含有0.2mg/L KT和2 g/L水解酪蛋白的UM培养基上,得到了最大的胚状体诱导率;水解酪蛋白的加入提高了胚状体的质量和成熟率.Medicago sativa L. cv. Baoding was used as material. The present study was included to identify the genotype of explant, the choice of media, endocrine and casein hydrolysate, petiole was better than cotyledon as expants. The improved SH medium was a better effective medium for the callus induction compared to UM medium. The maximal percent of the somatic embryo per callus was observed on UM medium with 0.2 mg/L KT and 2 g/L casein hydrolysate. Treatment with casein hydrolysate improved the quality and maturation of the embryo.

关 键 词：苜蓿 外体 愈伤组织 胚状体 再生 生根 

分 类 号：Q943.1[生物学—植物学]